Glucokinase I/G ratio change synthesis
Yes, insulin ^
Glucokinase pathway
Sugar trap
Glucokinase activity- add’l notes
High Km, low Vmax- fast w/low affinity
Hexokinase pathway
Sugar trap
Hexokinase- I/G ratio change synthesis?
Yes, insulin ^
Hexokinase activity notes
Inhibited by G-6-P
Low Km, low Vmax
Phosphofructokinase-1 pathway
Glycolysis
PFK-1 allosteric activators
AMP
Fructose 2,6-bisphosphate
PFK-1 allosteric inhibitors
ATP, citrate
PFK-1 synthesis changes by I/G ratio?
Yes, insulin ^
PFK-1 other notes (2)
**Rate limiting
ATP consuming
PFK-1 reciprocal regulation
FBPase-1
Pyruvate kinase pathway
Glycolysis
Pyruvate kinase allosteric activators
Fructose 1,6-bisphosphate
feed forward
Pyruvate kinase synthesis change w/ I/G ratio?
Yes, insulin ^
Pyruvate kinase: I/G ratio changes phosphorylation?
and how?
Yes
Insulin ^ dephosph ^
Glucagon/Epi ^ phosph ^
Pyruvate kinase & energy
ATP generating
Lactate dehydrogenase: pathway
Glycolysis/ anaerobic glycolysis
Pyruvate carboxylase: pathway
Gluconeogenesis
Pyruvate carboxylase allosteric activator
Acetyl CoA
Pyruvate carboxylase requires ___ for coenzyme act.?
Biotin
PFK-1 rxn?
Fructose-6-phosphate –> F 1,6 BisP
PDHC inhibitors
Acetyl CoA and NADH
PEP carboxykinase: pathway
Gluconeogenesis
Lactate dehydrogenase rxn?
Pyruvate –> Lactate
anaerobic glycolysis in cytosol
Pyruvate dehydrogenase rxn?
Pyruvate –> Acetyl CoA
in mitochondria
Pyruvate carboxylase rxn?
Pyruvate –> Oxaloacetate (OAA)
in mitochondria
PEP carboxylase: I/G change synthesis?
Yes, glucagon ^
