Unit 2 - Metabolic Processes Flashcards
(42 cards)
metabolism
catabolic and anabolic processes in a cell or organism
- catabolism: breakdown of molecules (e.g. starch→glucose→CO2 + H2O
- anabolism: building up of molecules (e.g. amino acids→proteins)
1st Law of Thermodynamics
Energy cannot be created or destroyed, but can only be converted from one form to another.
bond energy
the minimum amount of energy require to break one mole of bonds
exothermic reactions
- energy is released
- ΔH = negative
- increase in temperature of the solution
endothermic reactions
- energy is absorbed
- ΔH = positive
- decrease in temperature of the solution
enthalpy
measurement of heat (ΔH)
entropy
- measurement of randomness in energy (ΔS)
- as entropy increases, chaos increases
2nd Law of Thermodynamics
Over time, entropy increases (ΔSuniverse > 0).
free energy
- a.k.a. Gibbs Free Energy
- measurement of energy that can do useful work (ΔG)
- if ΔG = 0, the reaction is in equilibrium
exergonic reactions
- spontaneous (ΔG = negative)
- tend to be exothermic
- e.g. explosions
endergonic reactions
- non-spontaneous (ΔG = positive)
- tend to be endothermic
- e.g. photosynthesis
REDOX agents
molecules that facilitate energy transfer
endosymbiotic theory
- mitochondria evolved from prokaryotes
- proof of this is in its double membrane and its own DNA/RNA (i.e. mitochondrial RNA (mtRNA))
cellular respiration
C6H12O6 + 6O2→ CO2 + H2O
- can be anaerobic (no O2 required) or aerobic (O2 required)
- exergonic
- exothermic
- energy is released or stored as ATP
- made up of four stages:
- glycolysis
- pyruvate oxidation
- Krebs cycle
- electron transfer and chemiosmosis
- prokaryotes only undergo glycolysis
- eukaryotes undergo all four stages
POOPY I POOPY DING-DONG PROPER POOPY I CAN’T POOPY
glycolysis
- anaerobic
- occurs in cytosol
- biochemical pathway made up of 10 reactions:
1. phosphorylation
2. isomerization
3. phosphorylation
4. decomposition
5. decomposition
6. phosphorylation + REDOX
7. phosphorylation
8. isomerization
9. condensation
10. phosphorylation
major products/glucose:
- 2 net ATP
- 2 pyruvate
- 2 NADH
pyruvate oxidation
- occurs in intermembrane space
- reaction is doubled (2 pyruvate/glucose)
- biochemical pathway made up of 3 reactions:
1. decarboxylation
2. REDOX
3. synthesis
major products/glucose:
- 2 CO2
- 2 NADH
- 2 acetyl-CoA
Krebs cycle
- occurs in matrix
- reaction is doubled (2 acetyl-CoA/glucose)
- cyclical biochemical pathway made up of 8 reactions:
1. synthesis
2. isomerization
3. decarboxylation + REDOX
4. decarboxylation + REDOX + synthesis
5. decomposition + indirect phosphorylation
6. REDOX
7. hydration
8. REDOX
major products/glucose:
- 6 NADH
- 4 CO2
- 2 ATP
- 2 FADH2
DIG UNDER BIG COWS ONCE CONNOR, OK?
electron transport chain
(ETC)
- occurs in intermembrane space
- electron transfer and pumping of H+ from matrix into intermembrane space via cytochromes, and then through ATPase complexes to innermembrane space
- biochemical pathway made up of 5 cytochromes:
1. NADH dehydrogenase
2. ubiquinone (Q)
3. cytochrome b-c1 complex
4. cytochrome C (C)
5. cytochrome oxidase complex - final product is sweat (H+ + e- = H2O)
NADH → 3 H+
FADH2 (and NADH from glycolysis) → 2 H+
chemiosmosis
- establishment of chemiosmotic gradient; electrochemical potential
- H+ synthesizes the phosphorylation of ADP to ATP (1 H+ = 1 ATP)
factors that affect ATP synthesis
1. O2 availability
- O2 depletion
- e.g. coronary heart disorder, ischemia
2. glucose availability
- source of NADH and electrons
3. metabolic genetic disorders
- e.g. non-functional cytochromes
4. exposure to toxins, poisons, etc.
- e.g. cyanide binds with cytochromes
5. membreane permeability to NADH/FADH2, or H+
total theoretical yield of ATP
2 net ATP (glycolysis) + 2 ATP (Krebs cycle) + 32 ATP (ETC and chemiosmosis) = 36 ATP
total experimental yield of ATP
34 ATP
regulation of cellular respiration
- more ATP inhibits phosphofructokinase
- more ADP activates phosphofructokinase
- less citrate activates phosphofructokinase
- more NADH inhibits pyruvate decarboxylase
alcohol fermentation
- found in bread-making (yeast) and wine-making
1. glycolysis
2. 2 pyruvate → CO2 + 2 acetaldehyde
3. 2 acetaldehyde → 2 ethanol
major products/glucose
- 2 ATP
- 2 CO2
- 2 ethanol (EtOH)