Flashcards in Unit 6 - TCA Deck (12):
what percentage of ATP is produced under aerobic conditions?
over 90% of ATP
-due to TCA and oxidative phosphorylation in mitochondria
where are mitochondria most plentiful and where are they not?
most plentiful in cells with high E needs
not in mature erythrocytes
how much do mitochondria count for in liver cells?
up to 20% of total PRO
where does TCA happen compared to oxidative phosphorylation?
OP: embedded in inner membrane facing matrix
how is heavy traffic between cytoplasm and matrix space of mitochondria controlled?
controlled by specific transport systems except for O2 and CO2, which diffuse thru membrane
-pyruvate enters from cytoplasm to matrix
-cotransport for "X" (from matrix to cytoplasm) and "X"H2 (from cytoplasm to matrix)
--from NAD+ --> NADH RXN
-ATP/ADP cotransport (ATP from matrix to cytoplasm)
-Pi from cytoplasm to matrix
is there a transport system for NADH in the matrix?
-reducing equivalents from glycolytic NADH are transferred by indirect processes to matrix space or respiratory-chain component that faces intermembrane space
what are the 3 main functions of the TCA?
1. convert number of different fuels to a common mobile fuel (NADH)
2. serve as final meeting place for nearly all oxidizable substrates
3. provide intermediates for biosynthesis
how does the mechanism of succinyl-CoA synthetase illustrate the common intermediate principle?
S~CoA + Pi + E E-S~P + CoA
E-S~P E~P + succinate
E~P + GDP E + GTP
(where ~ means E-rich)
E~P is phosphohistidyl to couple exergonic RXN to endergonic RxN
what is the pyruvate dehydrogenase complex comprised of and located?
in the mitochondrial matrix space to oxidize pyruvate to ACoA (connecting link between glycolysis and CAC)
1. pyruvate dehydrogenase (E1)
2. dihydrolipyl transacetylase (E2)
3. dihydrolipyl dehydrogenase (E3)
what is the importance of lipoamine's structure?
it's a swinging arm, meaning kinetics are fast
-E2 can create multiple lipoamides
what is the main site of action for arsenite poisoning?