Urobilinogen/Bromsulfonthalein (BSP) BYE Extraction Test

Question 1

Colorless end product of bilirubin metabolism that is oxidized by intestinal flora to urobilin

Answer 1

Urobilinogen

Question 2

T/F. Urobilin is also photosensitive

Answer 2

True

Question 3

Urobilinogen Collection of sample:

Answer 3

Avoid light exposure

Question 4

Urobilinogen Specimen:

Answer 4

2-hr freshly collected urine or freshly collected stool

Question 5

Method use in Urobilinogen

Answer 5

Ehrlich’s Method

Question 6

Ehrlich’s Method Reagent:

Answer 6

P.DABA (p-dimethylaminobenzaldehyde)

Question 7

Urobilinogen Reference value:

Answer 7

1 Ehrlich = 1mg of urobilinogen

Question 8

Urine:

Answer 8

0.1-1.0 Ehrlich unit/2hour or 0.54 Ehrlich unit/day urine

Question 9

Stool:

Answer 9

75-275 Ehrlich unit/100g of feces

Question 10

Complete Biliary Obstruction =

Answer 10

Decreased urobilinogen

Question 11

T/F. Normally, B2 → intestine → urobilinogen

Answer 11

True

Question 12

In complete biliary obstruction, B2 will not be oxidized to urobilinogen =

Answer 12

Decreased amount or absent urobilinogen

Question 13

Bromsulfonthalein (BSP) BYE Extraction Test Principle:

Answer 13

addition of dyes/injection of dye (as waste) in the body

Question 14

Test for hepatocellular function and potency of bile duct

Answer 14

Bromsulfonthalein (BSP) BYE Extraction Test

Question 15

Rarely used because it utilize bromsulfonthalein dye which does not have any physiologic function since it is only a stain

Answer 15

Bromsulfonthalein (BSP) BYE Extraction Test

Question 16

2 administration:

Answer 16

Rosenthal White Method
Mac Donald Method

Question 17

Double collection method

Answer 17

Rosenthal White Method

Question 18

Rosenthal White Method BSP Dose:

Answer 18

2mg/kg body weight

Question 19

Rosenthal White Method Specimen collection: Blood (2)

Answer 19

o 5 mins after BSP administration
o 30 mins after BSP administration

Question 20

Rosenthal White Method Reference value:

Answer 20

o After 5 minutes: 50% dye retention
o After 30 minutes: 0% dye retention

Question 21

Single Collection method

Answer 21

Mac Donald Method

Question 22

Mac Donald Method BSP Dose:

Answer 22

5mg/kg body weight

Question 23

Mac Donald Method Specimen collection:

Answer 23

after 45 mins

Question 24

Mac Donald Method Reference value:

Answer 24

After 45 minutes: +/- 5% dye retention (slightly more or less than 5%)