Zoonoses 2 - Pasteurella, Bartonella, Leptospira Flashcards Preview

Bacteriology Lectures > Zoonoses 2 - Pasteurella, Bartonella, Leptospira > Flashcards

Flashcards in Zoonoses 2 - Pasteurella, Bartonella, Leptospira Deck (33):
1

Pasteurella organism and staining

Minute gram negative coccobacilli

bipolar staining (but hard to see)

2

Pasteurella metabolism and types

Facultative anaerobe, fastidious

Grows rapidly

4 capsular antigen types, A is the most common in humans

3

Pasteurella clinical presentation

local or disseminated edematous soft-tissue abscess

rapidly progressive cellulitis

*Proximal streaking along a lymph track

4

Pasteurella-related ____ is rare

PNA

5

Pasteurella is ______ that becomes an opportunistic pathogen

normal flora

6

Pasteurella animal clinical

fowl cholera (PNA) 

shipping fever (cattle) - septicemia

snuffles (rabbits) --> Hemorrhagic PNA

7

Pasteurella spread

  • Animal bites  (90% of cats, 70% of dogs)
  • Fomites occasionally

8

Pasteurella main virulence factor

What are the other ones?

Main = antophagocytic capsule

Others = pili, adhesins, Iron-Binding proteins

 

9

Pasteurella ___ Macrophages (hyaluronic acid capsule)

adhere to

(are NOT engulfed)

10

Pasteurella in vivo/vitro growth are...

equally fast

11

Pasteurella susceptibility to ABX

low b/c intracellular

12

Pasteurella ABX

pen/amp

or

ceph + tetracycline

13

Remember, most animal bite infections are...

polymicrobial

14

Bartonella organism

Looks like...

Gram negative rod (small)

Looks like rickettsia, but grows on lab media

15

Bartonella attachment protein attaches to 

feline RBC's

spread by fleas

16

Bartonella is ____ in humans but not RBC's

intracellular

17

Bartonella henselae disease

Cat Scratch Disease

  • papule at inoculation site
  • swelling and fever
  • Regional lymph node involvement
  • culture-negative endocarditis

18

Bartonella quintana disease

Trench fever (homeless fever)

= 5 day recurring fever, headache, lymphadenopathy

19

Disease caused by all types of Bartonella (including bacilliformis)

Bacillary angiomatosis

  • Higher incidence in AIDS patients
  • proliferates in small spherical blood vessels in an inflamed endothelial background
  • papular / tumoral lesion

 

20

Bartonella henslae transmitted by

cat scratches or bites

**#1 cause of localized lymph swelling in US children

21

Bartonella quintana spread by

human head and body lice

20% homeless infection rate

22

Bartonella pathogenic factors

  • Outer membrane binding protein
  • Penetration of endothelial cells (T4SS + Effector protein)
  • Stimulate NF-kB production and induce apoptosis by producing CSF

23

Bartonella treatment

(often none for cat scratch disease)

 

Azithromycin +/- Rifampin for bacillary angiomatosis

24

  • Leptospira interrogans shape
  • Growth
  • Energy requirement
  • Visualization

  • Spirochete (thin) with Endoflagella
  • Easily grown (6 days)
  • ALL ENERGY from LCFA's
  • Hard to see with Dark Field, better with Scanning EM

 

25

Leptospira  clinical - stages?

initial and second stage

Initial

  • fever (bacteremia)
  • capillary vasculitis and edema organs (LEAK RBC and serum = petechiae)

Secondary (10%) 

  • Jaundice from liver failure
  • Nitrogen retention from kidney medulla destruction
  • Bloodshot eyes
  • Meningitis (rare)
  • Nausea and vomiting from organ fail

26

Secondary leptospirosis a.k.a. 

Weil's disease

27

Initial Leptospira bacteremia does not hit...

liver, kidney, CNS, eyes

28

Leptospira shed via...

Acquired via...

Also spread via...

  • infected kidney shedding into water (organism can persist for weeks)
  • Ingesting contaminated water
  • Animal urine (rats + dogs)

29

Leptospira toxin

none! just LPS and PG

30

Leptospira ____ growth is possible

intracellular

31

Leptospira pathogenesis is primarily due to ______

______ organs are more infected.

due to endothelial damage in capillaries

Highly perfused organs

32

Leptospira ABX effective?

Which ones?

Prophylaxis?

  • Limited use because disease is mostly inflammatory (give corticosteroids)
  • Macrolides or quinolones
  • Doxy for prophylaxis

33

Other Leptospira control measures (2)

avoid rat-contaminated water

vaccinate dogs with distemper-hepatitis-leptospirosis vaccine