(1) The Laboratory in Diagnosis

Question 1

Haematoxylin stains which areas blue

Answer 1

Acidic

Question 2

Eosin stains which areas pink

Answer 2

Basic

Question 3

Haematoxylin stain what colour

Answer 3

Blue

Question 4

Eosin stains what colour

Answer 4

Pink

Question 5

The bladder is known as a ____ site

Answer 5

Sterile

Question 6

The perineum is known as a ____ site

Answer 6

Non-sterile

Question 7

Name two factors that affect blood culture sensitivity

Answer 7

1. Volume of culture taken

2. If someone has already had antibiotics

Question 8

What organisms can you see by light microscopy

Answer 8

Extracellular bacteria with a cell wall

Question 9

Gram negative organisms will be what colour

Answer 9

Pink

Question 10

Gram positive organisms will be what colour

Answer 10

Purple

Question 11

Ziehl-Neelsen stain is used for

Answer 11

Mycobacteria (acid fast)

Question 12

Ziehl-Neelsen stain will be what colours

Answer 12

Blue background, red bacteria

Question 13

India - ink stain is used for

Answer 13

Cryptococcus

Question 14

Giemsa stain is used for

Answer 14

Malaria

Question 15

A positive catalase result is

Answer 15

Hydrogen peroxide bubbles (staph +ve, strep -ve)

Question 16

A positive coagulase is

Answer 16

White clumps (aureus +ve, epidermidis -ve)

Question 17

What can you find out from a MALDI-TOF test

Answer 17

Mass spectrometry identifies protein components of bacteria to find protein ID

Question 18

Percentage of sick people correctly identified as having the disease =

Answer 18

Sensitivity

Question 19

Percentage of healthy people correctly identified as not having the disease =

Answer 19

Specificity

Question 20

Probability disease is present if the test is positive =

Answer 20

Positive predictive value

Question 21

Probability disease is absent if the test is negative=

Answer 21

Negative predictive value