Flashcards in 1.1.1 - Cell Structure (Microscopy) Part 1 & 3 Deck (10):
State the resolution and magnification that can be achieved by a light microscope.
Resolution - 200nm
Magnification - x1,500
State the resolution and magnification that can be achieved by a transmission electron microscope.
Resolution - 0.2nm
Magnification - x500,000
State the resolution and magnification that can be achieved by a scanning electron microscope.
Resolution - 0.2nm
Magnification - x100,000
Explain the difference between magnification and resolution.
Magnification - The number of times larger an image is
Resolution - The ability to distinguish between two separate points
Explain what staining (in microscopy) is.
Staining refers to any process that helps to reveal or distinguish different features in a sample.
Explain the need for staining samples when using a light microscope.
Many biological materials are not coloured, so you cannot see any details. Material may also become distorted when being cut into sections. Coloured stains are chemicals that bind to other chemicals on or in the specimen, allowing for the specimen to be seen.
Explain the need for staining samples when using a transmission electron microscope.
The final image produced from an electron microscope is always in black, white and grey. Colours are added using a specialised computer software to reveal any features in the sample that may not be as visible as a greyscale image.
What is the difference between a TEM and an SEM? List 3 differences.
- Electron beam passes through the thin sample
- Final image is 2D
- Magnification = x500,000
- Electron beam bounces off the sample
- 3D image of the surface is produced
- Magnification = x100,000
List some advantages of an electron microscope.
- The resolution is 1000x more than light microscope.
- They can produce detailed images of cell organelles.
- SEM produces 3D images which can reveal cellular or tissue arrangements.