Protein Transport + Secretion Flashcards

1
Q

How many proteins does the cell membrane have?

A

300

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2
Q

How many proteins does the outer membrane have?

A

50

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3
Q

How many proteins does the periplasm have?

A

100

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4
Q

T4S

A

Conjugate pilus

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5
Q

Fla/T3S

A

Effector/toxins

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6
Q

CU

A

Pilus

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7
Q

T2S

A

Hydrolytic enzymes, toxins, type IV pilus

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8
Q

Components of Sec system

A

-Leader peptide on preprotein (SIGNAL)
- Signal recognition particle (SRP)

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9
Q

Preprotein

A

Not in shape yet

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10
Q

Cleavage site

A

leader peptide cleaved once transported across membrane

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11
Q

N-terminal

A

start of protein/peptide
–> recognizes leader
–> trafficks to SecYEG to interact and be transported across membrane

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12
Q

Chaperone

A

SecB

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13
Q

Membrane complex

A

SecYEG

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14
Q

ATPase

A

SecA

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15
Q

SecB 2 modes of binding

A

Premiscuous binding to polypeptide
- specific binding partner (SecA)

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16
Q

What happens during promiscuous binding to polypeptide?

A
  • keeps protein in translocation competent state
  • prevent from aggregation + folding before IM
17
Q

What happens during specific binding to SecA

A
  • delivers preprotein to SecA, engages with SecB
18
Q

SecYEG

A
  • membrane translocase
  • pore in hydrophyllic channel
  • allows delivery to periplasm or IM
19
Q

Goal of SecYEG

A

deliver mature protein to periplasm in unfolded state

20
Q

Charge of N-domain, C-terminal and H-domain

A

N - basic charge
C - neutral
H - hydrophobic region

21
Q

2 options for prepeptide

A
  1. stay in membrane
  2. released to periplasm
22
Q

TAT

A

Twin-agenine translocation