1.4 Factors affecting enzyme activity

Question 1

name the 4 factors that may affect enzyme activity

Answer 1

• temperature
• pH
• substrate concentration
• enzyme concentration

Question 2

what will occur in extreme temp

Answer 2

denature

Question 3

Effect of temp on enzyme controlled reactions:

Answer 3

• as temp increases, rate of reaction increases
• more kinetic energy = more successful collisions
• more enzyme substrate complexes formed
• past optimum temp, rate decreases

Question 4

why do enzymes denature due to high temp

Answer 4

• gain so much kinetic energy that tertiary structure bonds break
• looses 3D shape ∴ no successful collisions, ∴ no enzyme substrate complexes

Question 5

Effect of pH on enzyme controlled reactions

Answer 5

• as pH becomes more acidic, rate decreases
• more H+ present will disrupt active site
• as pH becomes more alkaline, rate decreases
• more OH- ions present will disrupt active site

Question 6

effect of increased substrate concentration

Answer 6

• increases substrate conc = increases rate
  OR if all active sites are occupied rate stays constant

Question 7

effect of increased enzyme concentration

Answer 7

• increasing enzyme conc = increase rate OR if all substrate molecules are occupying active sites, rate stays constant