Eukaryotic gene promotors

Question 1

What are promotors?

Answer 1

Genomic regions where RNAP binds
-complex in euks

Question 2

How is transcription controlled in eukaryotes?

Answer 2

-cis regulatory elements
-DNA binding proteins

Question 3

What are the two classes of promotors?

Answer 3

Highly inducible promotors
-contain TATA box (close to transcription start site)
Constitutively expressed promotors
-transcribed at multiple start sites from CpG islands (CG clusters) (N/B: methylation of CpG islands represses transcription)

Question 4

How are transcription regulatory elements organised in mammalian genes?

Answer 4

TATA box promotor upstream near transcription start site
Promotor-proximal elements 200nts upstream of transcription start site
Enhancers and silencers up or down stream of promotor

Question 5

How are transcription regulatory elements organised in yeast genes?

Answer 5

TATA box promotor upstream near transcription start site
Upstream activating or repressing sequence (UAS/URS)