Cytoprep and Cytology Staining Flashcards
1
Q
What type of stain is the DiffQuik?
A
Romanowsky. It is a faster version of the Wright Giemsa, using 3 solutions: Sol1 methanol (often with Malachite Green); Sol2 xanthene dye (usually Eosin y); Sol3 methylene blue and Azure A
2
Q
What is the main stain for cytology?
A
The Papanicolaou or Pap stain.
3
Q
How many dyes are used in the Pap stain?
A
Five. Hematoxylin, Orange G, Eosin, Light green, and Bismark brown.
4
Q
True or False: EA is a polychromatic dye.
A
True. It consist of Eosin, Light Green, and Bismarck Brown.
5
Q
How is the Pap stain different from FFPE special stains?
A
The preferred fixative is alcohol, it was developed for demonstrating cells from body fluids rather than tissue, and it is meant to stain transparently.
