2.2.3 Hematoxylin and Eosin Staining (SEM) Flashcards by Miguel Acosta | Brainscape

Q

H & E staining category?

A

Histological

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Q

H & E staining Method?

A

Regressive

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Q

Nuclear Stain
Basic Dye
Impart Blue color

A

Hematoxylin

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Q

Cytoplasm stain
Acid dye
Impart pink color

A

Eosin

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Q

Procedure of H&E staining:

For Further deparaffinization

a. 2 changes of Xylene
b. Descending grades of alcohol
c. Hematoxylin
d. Acid alcohol
e. Ammonia water
f. Eosin
g. Ascending concentrations of alcohol
h. 2 changes of Xylene

A

a

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Q

Procedure of H&E staining:

Nuclear staining

a. 2 changes of Xylene
b. Descending grades of alcohol
c. Hematoxylin
d. Acid alcohol
e. Ammonia water
f. Eosin
g. Ascending concentrations of alcohol
h. 2 changes of Xylene

A

c

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Q

Procedure of H&E staining:

Hydration

a. 2 changes of Xylene
b. Descending grades of alcohol
c. Hematoxylin
d. Acid alcohol
e. Ammonia water
f. Eosin
g. Ascending concentrations of alcohol
h. 2 changes of Xylene

A

b

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Q

Procedure of H&E staining:

Decolorizing

a. 2 changes of Xylene
b. Descending grades of alcohol
c. Hematoxylin
d. Acid alcohol
e. Ammonia water
f. Eosin
g. Ascending concentrations of alcohol
h. 2 changes of Xylene

A

d

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Q

Procedure of H&E staining:

Blueing agent

a. 2 changes of Xylene
b. Descending grades of alcohol
c. Hematoxylin
d. Acid alcohol
e. Ammonia water
f. Eosin
g. Ascending concentrations of alcohol
h. 2 changes of Xylene

A

e

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Q

Procedure of H&E staining:

Counterstain / Cytoplasmic

a. 2 changes of Xylene
b. Descending grades of alcohol
c. Hematoxylin
d. Acid alcohol
e. Ammonia water
f. Eosin
g. Ascending concentrations of alcohol
h. 2 changes of Xylene

A

f

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Q

Procedure of H&E staining:

For Clearing prior to mounting

a. 2 changes of Xylene
b. Descending grades of alcohol
c. Hematoxylin
d. Acid alcohol
e. Ammonia water
f. Eosin
g. Ascending concentrations of alcohol
h. 2 changes of Xylene

A

h

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Q

Procedure of H&E staining:

Dehydration

a. 2 changes of Xylene
b. Descending grades of alcohol
c. Hematoxylin
d. Acid alcohol
e. Ammonia water
f. Eosin
g. Ascending concentrations of alcohol
h. 2 changes of Xylene

A

g

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Q

Forms of hematoxylin used in exfoliative cytology and for staining of sex chromosomes

A

Harris Hematoxylin

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Q

Form of hematoxylin used of immunohistochemistry

A

Mayer’s Hematoxylin

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Q

Form of hematoxylin used to demonstrate spermatogenesis

A

Copper hematoxylin

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Q

Component of dye responsible for coloring property

A

Chromophore

Q

Components of the dye responsible for dyeing property (retain)

A

Auxochrome

Q

Dyes without auxochrome component

Also called oil soluble dyes

Used as fat stains

SUDAN dyes

A

Lysochrome