Methodology

Question 1

light microscopy

Answer 1

thin sections of tissue; can e used on live or fixed and stained cells; white light or transmitted light

Question 2

resolution

Answer 2

amount of detail/info retained from the specimen upon magnification

Question 3

fluorescent light microscopy

Answer 3

same limit of resolution as white light microscopy
can observe fluorophores even in live cells
high contrast

Question 4

FRET

Answer 4

Fluorescent Resonance Energy Transfer
good for determining:
1. how close in a cell two molecules are
2. to follow the activation of an enzyme in situ

Question 5

laser scanning confocal microscopy

Answer 5

similar to fluorescent microscopy, but uses lasers as illumination and can only visualize one focal plane at a time
larger specimens

Question 6

FRAP

Answer 6

Fluorescence recovery after photobleaching

Question 7

scanning electron microscopy (SEM)

Answer 7

reveals specimen topography

specimens are dehydrated then metal coated

Question 8

max resolution of SEM

Answer 8

5nm

Question 9

transmission electrom microscopy (TEM)

Answer 9

very high resolution images

Question 10

problem with TEM

Answer 10

potential for artifacts

Question 11

resolution of TEM

Answer 11

0.1nm-2nm

Question 12

cell culture

Answer 12

growing cells outside of the organism

Question 13

differential centrifugation

Answer 13

homogenize cells and isolate some organelles which can then be separated from one another through sub-cellular fractionation