What is the high energy state between substrate and product?
What is the minimum energy required for reaction to take place called?
How do enzymes work broadly?
Lower activation energy - facilitate transition state
What does the Michaelis- Mentes model say
That doubling enzyme doubles rate of reaction (with infinite substrate).
Whats the max rate of reaction when all ES complexes are taken? What does it define?
Defines rate of reaction
Conc of substrate that gives half Vmax (lower better)
Estimates affinity of enzyme for substrate
Covalent bonds form in which kind of enzyme inhibition
What is special about enzyme?
Unchanged post reaction
What two things can go wrong with enzymes?
Inherited genetic disorders
Somatic problems - cancer
What is a small region of enzyme of specific amino acid sequence where a substrate binds
Whats induced fit hypothesis
That binding of substrate causes conformational change in enzyme
Do weak or strong bonds hold substrate in enzyme?
What is the units for Vmax?
normally μM per L or per g
What the Lineweaver-Burk plot and how can you work out Km Vmax
It is the inverse graph of Michaelis-Mentes.
-1/Km intercepts X axis
1/Vmax intercepts Y axis
Does competitive enzyme inhibition affect Vmax or Km? Non-Competitive?
Competitive- affects Km not Vmax
Non-competitive affects Vmax not Km