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Flashcards in Enzymes Deck (15)
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1
Q

What is the high energy state between substrate and product?

A

Transitional

2
Q

What is the minimum energy required for reaction to take place called?

A

Activation Energy

3
Q

How do enzymes work broadly?

A

Lower activation energy - facilitate transition state

4
Q

What does the Michaelis- Mentes model say

A

That doubling enzyme doubles rate of reaction (with infinite substrate).

5
Q

Whats the max rate of reaction when all ES complexes are taken? What does it define?

A

VMax

Defines rate of reaction

6
Q

Whats Km

A

Conc of substrate that gives half Vmax (lower better)

Estimates affinity of enzyme for substrate

7
Q

Covalent bonds form in which kind of enzyme inhibition

A

Irreversible

8
Q

What is special about enzyme?

A

Highly specific

Unchanged post reaction

9
Q

What two things can go wrong with enzymes?

A

Inherited genetic disorders

Somatic problems - cancer

10
Q

What is a small region of enzyme of specific amino acid sequence where a substrate binds

A

Active site

11
Q

Whats induced fit hypothesis

A

That binding of substrate causes conformational change in enzyme

12
Q

Do weak or strong bonds hold substrate in enzyme?

A

Weak

13
Q

What is the units for Vmax?

A

normally μM per L or per g

14
Q

What the Lineweaver-Burk plot and how can you work out Km Vmax

A

It is the inverse graph of Michaelis-Mentes.

-1/Km intercepts X axis
1/Vmax intercepts Y axis

15
Q

Does competitive enzyme inhibition affect Vmax or Km? Non-Competitive?

A

Competitive- affects Km not Vmax

Non-competitive affects Vmax not Km