Gram Positive Cocci Flashcards
Staph epidermidis
Gram Stain and Colony descriptions
GPC in Loose clusters and in tetrads
Staphylococcus epidermidis
Growth on media
Small to medium, Smooth, raised, entire, gray-white
Staphylococcus epidermidis
Tests
Catalase positive Coagulase negative Novobiocin Sensitive DNase negative (no zone of inhibition) Mannitol fermentation negative (remains pink)
Staph aureus
Gram Stain
GPC in clusters
Staph aureus
Growth on media
Medium to large, Smooth, raised, entire, golden yellow
Staph aureus
Tests
Catalase pos Coagulase pos Gelatin hydrolysis pos DNase pos Mannitol fermentation pos (turns yellow)
Staph saprophyticus
Gram Stain
GPC in pairs or tetrads
Staph saprophyticus
Growth on media
Large, convex, entire, white (can be yellow or orange), glossy
Staph saprophyticus
Tests
Catalase pos
Coagulase neg
Novobiocin resistant
Urease pos
Strep pyogenes
Gram Stain
GPC in chains
Strep pyogenes
Growth on media
Beta hemolytic, grayish-white
Strep pyogenes
Tests
Catalase neg
Beta hemolytic
PYR pos
Bacitracin Susceptible
Strep agalactiae
Gram Stain
GPC in chains
Strep agalactiae
Growth on media
Beta hemolytic, flat, glossy
Strep agalactiae
Tests
Catalase neg Beta hemolytic Hippurate pos CAMP pos Carrot broth pos
Strep pneumoniae
Gram Stain
GPC, lancet-shaped in pairs, intra or extracellular
Strep pneumoniae
Growth on media
Alpha hemolytic, small, grey, dimpled, can be mucoid
Strep pneumoniae
Tests
Catalase neg Alpha hemolytic Optochin pos (>14mm) Bile solubility pos Quellung reaction pos (capsule swells)
Alpha Viridans
Gram Stain
GPC in long chains
Alpha viridans
Growth on media
Minute to small, gray, domed, alpha hemolytic
Alpha viridans
Tests
Catalase neg
Alpha hemolytic
Optochin neg
Bile solubility neg
Enterococcus
Gram Stain
GPC in short chains and pairs
Enterococcus
Growth on media
Small, cream or white, entire, gamma hemolytic
Enterococcus
Tests
Catalase neg Gamma hemolytic Bile esculin pos PYR pos 6.5% NaCl pos
