Lecture 8 Flashcards
1
Q
How to ensure you are getting proper staining?
A
control
2
Q
atrichous
A
no flagella
3
Q
endospores
A
- a dormant daughter cell formed in the main cell
- resists drying out, heat, chemicals, radiation
- not “alive”
4
Q
what kills endospores
A
autoclaves
5
Q
most notable endospore forming groups
A
bacillus and clostridium
6
Q
example of bacillus
A
anthrax, insecticides
7
Q
examples of clostridium
A
tetanus, botulism, gangrene
8
Q
endospore stain
A
- use moist heat to stain endospore wall
- malachite green onto slide over a steaming water bath for 5 mins
- rinse with water
- counterstain with safranin
9
Q
aseptic technique
A
- prevention of contamination when cultivating microorganisms
- containment of organisms ur working with
10
Q
most common way to transfer bacteria
A
loop
11
Q
most common way to keep things sterile and reduce airflow
A
flame
12
Q
in aseptic technique, plates should be kept…
A
inverted and open only when actively used
13
Q
bacterial growth
A
number not size
14
Q
physical factors that affect growth
A
- temp
- pH
- salinity
- nutrients
15
Q
mesophiles
A
cause disease b/c they optimally grow at body temp
