Centrifugation Flashcards
Homogenisation
Breaking up and mixing of the material together uniform preparation. The marginalisation can be carried out using a mortar and pesto or a blender
Centrifugation
Place in the preparation and sent a few tubes and spending them in a centrifuge at high speed. The forces involved, centrifugal forces, will cause the large heavier particles to settlement with the smaller lighter particles remaining in suspension at the top in the supernatant
Order of sedimentation
Cell debris Nuclei Chloroplast Mitochondria Lysosomes Fragments of cell membrane and RER Ribosomes
What is important when setting up a centrifuge
Each centrifuge Tube is counterbalanced by a tube containing the same volume of material
What Will alter been the speed of rotation and with the time of centrifugation do
Make it possible to isolate and sediment particular organelles or cell components. If the process is repeated progressively light organelles or sediment in sequence
Why use a stain
Differential my stain cell components to aid viewing
