Agarose Gel Electrophoresis

Question 1

2 Components of 6X gel loading buffer for DNA age

Answer 1

.0100g bromophenol blue
1.6 g sucrose

Question 2

3 Components of 1X Tris-Acetate-EDTA (TAE) running buffer

Answer 2

4.84g Tris
1.14 glacial acetic acid
2 ml of 0.5 EDTA ph 8.0

Question 3

Thickness of agarose gel and number of well combs

Answer 3

3-5 mm
10 well combs
Add electrophoresis buffer to cover gel 2 mm depth when set

Question 4

Etbr meaning and color

Answer 4

Ehtidium bromide
Dark red

Question 5

How to take photo of gel

Answer 5

UV transilluminator

Question 6

6X gel loading dye color

Answer 6

Purple bc mixture of red and blue
But in the experiment the components are bpb and sucrose so i guess only blue

Question 7

DNA loading dye

Answer 7

One tracking dye (BPB) and high density reagent (sucrose)

Question 8

Tracking dye

Answer 8

Track progress of gel

Question 9

High density reagent

Answer 9

Increase density to settle sample at the bottom of the well
Confine sample in the well without diffusing

Question 10

Bromophenol blue color

Answer 10

Ph 3.0 - yellow
Neutral - blue
Ph 4.6 - purple

Question 11

Bromophenol blue charge

Answer 11

Weak acid
Negative charge at neutral/slightly basic pH = migrate towards positive