Apoptosis Flashcards
1
Q
Intrinsic Pathway
A
Activation of BH3-only proteins Inhibition of pro-survival BCL2 proteins Activation of BAK/BAX - permeablize mitochondria Cytochrome c released into cytoplasm Binds to APAF Activates caspase 9 Activates caspase 3 and 7
2
Q
Extrinsic Pathway
A
TNFa binds to TNFR (or other activation of death-domain receptor family)
Activates CASP8
CASP 8 activates casp-3 and 7, and activates intrinsic pathway via tBID
3
Q
Apoptosis morphology
A
Cell shrinkage, membrane blebbing, release of apoptotic bodies
Removal by recognition of phosphotidylserine on extracellular surface
4
Q
Activation of apoptosis
A
Intrinsic pathway: DNA damage, lack of growth factor signalling, ER stress
Extrinsic pathway: TNFa binding or FasL binding to Death-domain receptor family
