Assessed practical Flashcards
1
Q
Where are gloves disposed?
A
Yellow bin with yellow bag
2
Q
Why would you have more than one colony type?
A
Working too far from Bunsen burner
Leaving the lid off too long
Not flaming loop enough
3
Q
Why do you use one single bacterial strain/
A
Minimise genetic variation, to understand potential for disease and therefore health and safety implications
4
Q
Whats the chemical name for Ellman’s reagent?
A
5,5’ - dithiobis (2-nitrobenzoic acid)
5
Q
Describe DTNB
A
Used to quantify SH gross, react with SH groups to produce TNB 2- which is yellow
Greater SH conc = greater absorbance of light
Plot absobrance=. conc on x acid and absorbance on y
