B3.6 Flashcards
1
Q
the effect of pH on the reaction of amylase
A
Placing several different starch solutions of a know volume and concentration in a water bath not higher than 37 degress celsius
Adding a buffer solution at a different pH to each starch solution
Setting up spotting tiles for each test solution with a drop of iodine in each well
Mixing the same volume and concentration of amylase into each tube
Starting a stop clock as soon as the enzyme is added
Taking samples every 30 seconds using a pipette and adding each sample to an iodine filled well
Observing and recording results that can be displayed graphically to compare the effect of pH on the rate of an amylase - catalysed reaction
