Bio topic 1 Flashcards
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what is diffusion
Diffusion is the movement of a substance from an area of high concentration to an area of lower concentration
what are the 5 structures of an animal cell
nucleus: contains genetic material to control a cells activities
cytoplasm: gel-like substance where chemical reactions happen and site of anaerobic respiration (contains enzymes)
cell membrane: holds the cells structure and control what moves in and out of cell
mitochondria: site of aerobic respiration so cells have energy
ribosomes: site of protein-synthesis
what are the 8 structures of a plant cell
it has:
nucleus: contains genetic material to control a cells activities
cytoplasm: gel-like substance where chemical reactions happen and site of anaerobic respiration (contains enzymes)
cell membrane: holds the cells structure and control what moves in and out of cell
mitochondria: site of aerobic respiration so cells have energy
ribosomes: site of protein-synthesis
but also:
rigid cell wall: strengthens and supports the cell, made of cellulose
permanent vacuole: contains cell sap (solution of salts/sugars)
chloroplast: contains chlorophyll for absorption of light t photosynthesize
what three structures differ from plant cells and animal cells
- chloroplast
- cell wall
- vacuole
what are the structures in a normal bacteria cell
BACTERIA DO NOT HAVE CHLOROPLAST OR MITOCHONDRIA NOR A NUCLEUS!!!
cytoplasm: gel-like substance where anaerobic respiration and other chemical reactions requiring enzymes happen
cell-membrane: holds cell together and controls what enters and exits
cell-wall: supports and strengthens cell
plasmids: rings of DNA that is NOT enclosed in a nucleus
single strand DNA: controls cells activities
what is a prokaryote
+ give examples
A prokaryotic cell is a type of cell that does not have a true nucleus or membrane-bound organelles.
e.g: bacteria and Archaea are prokaryotic cells
size: 0.2 μm – 2.0 μm
what is a eukaryote
+ give examples
A eukaryotic cell contains membrane-bound organelles such as a nucleus, mitochondria.
e.g: protozoa, fungi, plants, and animals.
size: 5 μm – 100 μm
what are the main differences of prokaryotes and eukaryotes?
- prokaryotes are smaller than eukaryotes
- a prokaryote doesn’t have a membrane-bound nucleus
- prokaryotic cells divide through binary fission whereas eukaryotes go through mitosis
- prokaryotes don’t have mitochondria or chloroplast
MICROSCOPY- experiment
- prepare glass/plastic microscope slide
- place drop of water in middle of clean slide
- using tweezers peel of epidermal tissue from onion and put it on slide
- add drop of iodine to stain cell (helps view results)
- place cover slip on top
- clip slide onto stage of light microscope
- look through eyepiece to see results (adjust for clearer view)
- draw what you see (cell structures) and label them
equation for magnification
image size / actual size
what is cell differentiation
when a cell changes to become specialised for their job
sperm cells function + adaptations
its job is to carry male DNA and fertilize egg (female DNA) for reproduction
- has flagellum = helping it swim
- has streamlined head = help it swim
- has lots of mitochondria = energy to swim
- enzymes (acrosomes) at head = digests through eggs cell membrane
- has nucleus = contains 23 chromosomes for fathers DNA
nerve cells functions + adaptations
carries rapid electrical signals around body
- have branched connections = connects to others nerves to form networks
- axons = long fibres to carry electrical impulses around body
- neurotransmitters = chemicals that crosses between synapses
- synapses = allows impulses to pass from one nerve cell to another
- myelin sheath insulates axons to speed up transmission of nerve impulses
- dendrites = increases SA for other nerve cells to connect to easily
muscle cells functions + adaptations
contracts and relaxes
- protein fibres = can change length of muscle ,fibres shorten to contract muscle
- many mitochondria = energy to contract through aerobic respiration
Root hair cells function + adaptations
absorbs water and minerals for plants
- has root hairs= increases SA for contact to soil water for osmosis and mineral ions through active transport
- thin walls = faster rate of diffusion
- don’t have chloroplast = root hair is underground so it would be ineffective at absorbing light
Phloem function + adaptations
for translocation: moves food substances that is produced by photosynthesis to where needed in plant (moves both up and down cell) and requires energy
- sieve plates = pores that allow sugars to move through cells interior helps transport
- phloem vessel cells has no nucleus and little cytoplasm so dissolved sugars move more effectively
- companion cells = provides energy for phloem vessel cells
xylem function + adaptations
transports water and soluble minerals from the roots up the plant stem and into the leaves
- Lose their end walls = so the xylem forms a continuous hollow tube for water to flow
- lignin = strengthen walls to support plant
- made of dead cells = makes xylem impermeable
- unidirectional
what are coiled up lengths of DNA called
chromosomes
half from mother and father
[there are 23 pairs of chromosomes, so 46 in total]
what are the female chromosomes
XX
what are the male chromosomes
XY
how is mitosis used to grow and repair damaged cells for two identical cells to original
[IPMATC]
interphase = (cell spends majority of time in this phase) chromosomes replicate and the proteins synthesize
prophase = DNA in chromosome its copy condenses to become visible. membrane around nucleus shrinks and disappears
metaphase = Chromosomes and their copies line up in the middle of the cell
anaphase = Chromosomes and their copies are pulled to different ends of the cell
telophase = chromosomes decondense and new membranes form around the chromosomes at each end of the cell to become nuclei of two new cells
cytokinesis = The cell membrane pinches in and eventually divides into two daughter cells.
process makes two identical new daughter cells with same DNA to parent cell
how do prokaryotic cells divide through the process of binary fission
prokaryotic cells replicate through binary fission
- circular DNA and plasmids replicate
- the cell gets bigger and DNA moves to opposite ends (poles) of cell
- cytoplasm divides and two new daughter cells which have one copy of circular DNA but vary in plasmids
how do u find number of bacteria in population
- convert given time into minutes
- total time stated/time for cell to divide = the number of division
- then put the number of divisions calculated as a power
e.g; 2.5 hours x 60 min = 150 min
150 min / 30 min = 5 divisions
2^5= 2 x 2 x 2 x 2 x 2 = 32 cells
how do u grow (culture) microorganisms in a lab
- bacteria is grown in a culture medium which contains carbohydrates, minerals, proteins, and vitamins for growth
- the culture medium can be solid agar jelly or a nutrient broth solution
-bacteria grown on agar plates will form visible colonies or spreads out on surface of the jelly
[to make an agar plate you pour hot agar jelly into shallow petri dish and once it cools and sets inoculating loops or a sterile dropping pipette with a spreader can transfer microorganism onto jelly]
in a school lab what temp should microorganism cultures be kept at and why
25 degrees C : harmful pathogens can grow above this temp
in industrial work what temp should microorganism cultures be kept at and why
incubated at higher temps for increased rate of growth
how do u investigate the effect of antibiotics/antiseptics on bacteria’s growth
1- place disk soaked in (differing types of antibiotics or differing concentrations) antibiotics on agar plate with space between discs
2- antibiotics diffuses (soaks) into agar jelly
3- as bacteria grows in petri dish, the zone of inhibition forms
(make sure u put a control paper disk to keep test fair)
4- leave plate at 25 degrees C for 48 hors
5- compare the results
how do you avoid contamination in the culturing microorganism experiment
sterilisation = clean petri dish by heating it at high temp to kill unwanted microorganisms
sterilise inoculating loop = pass it over hot flame so bacteria isn’t transferred
petri dish should be lightly taped to lid to stop unwanted microorganisms from entering in air
petri dish should be stored upside down = stops drops of condensation falling onto agar
how do you calculate the zone of inhibition
Area= π x radius^squared
what are stem cells
an undifferentiated cell (not yet gone through differentiation)
what are the two types of stem cells? [humans]
- embryonic stem cells
- adult stem cells