BIOL Lab 5: DNA

Question 1

DNA Extraction

Answer 1

Samples must be connected be collected and the DNA in those samples must be separated from the cells themselves in order to conduct an analysis on the pure DNA.

Question 2

DNA Cut with Restriction Enzymes

Answer 2

The DNA samples must be exposed to enzymes that cut the DNA at very specific places to produce an unique DNA fingerprint for each individual involved in the investigation.

Question 3

DNA Run on a Gel Electrophoresis

Answer 3

The cut DNA samples are put in a gel matrix and subjected to an electrical charge to separate out the DNA fragments by size. This produces a visual representation of the unique DNA fingerprints of the individuals.

Question 4

Restriction Enzymes

Answer 4

They “cut” the DNA sample at very specific base pair sites.