Biomedical Laboratory Techniques (5/14) Flashcards

1
Q

PCR requirements

A

DNA polymerase
deoxynucleotides
reaction budder
primer specific to target sequence

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2
Q

Steps of PCR

A

denature
annealing
extension- Taq polymerase

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3
Q

Sanger sequencing requirements

A

ddNTPs, DNA polymerase, dNTPs, fluorescent markers, gel electrophoresis apparatus

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