BIOTECHNOLOGY: PRINCIPLES AND PROCESSES

Question 1

EFB

Answer 1

EUROPEAN FEDERATION OF BIOTECHNOLOGY

Question 2

1st rDNA was founded by

Answer 2

Stanley Cohen + Herbert Boyer

Question 3

1st rDNA is

Answer 3

From Salmonella typhimurium

Cloned in E. coli

Question 4

Origin of replication / ORI

Answer 4

1. Sequence where replication starts

2. Responsible for controlling copy number

Question 5

Selectable marker

Answer 5

Identify + eliminate non -transformants

Question 6

Transformation

Answer 6

Introduction of a piece of DNA into host

Question 7

EX. of selectable markers ( 3)

Answer 7

1. Ampicillin
2. Chloramphenicol
3. Tetracycline/ kanamycin

Question 8

Insertional inactivation of gene for synthesis of beta galactosidase

Answer 8

Recombinants - no colour

Non - recombinants - blue colour

Question 9

Plasmid

Answer 9

Autonomously replicating circular extra chromosomal DNA

Question 10

Recognition site of EcoR1

Answer 10

G A A T T C

Question 11

Recognition site of Hind 3

Answer 11

A A G C T T

Question 12

Recognition site of BamH1

Answer 12

G G A T C C

Question 13

Recognition site of Sal1

Answer 13

G T C G A C

Question 14

Naming of Endo nuclease

Answer 14

1st letter - genus
2nd letter - species
3rd letter - strain
Roman number - order of extraction

Question 15

Endonucleases recognises

Answer 15

Palindromic nucleotide sequence

Question 16

No longer pathogenic / disarmed vectors

Answer 16

Plants - Ti plasmid
Animals - Retrovirus
Convert normal cells to cancerous

Question 17

DNA competent by treating with

Answer 17

Divalent cations.

Question 18

Heat shock

Answer 18

1. Ice
2. Heat
3. Ice

Question 19

DNA directly injected into nuclease

Answer 19

Microinjection

Question 20

Bombarded with high velocity micro marticles

Answer 20

Bio lipsticks / gene gun

Question 21

Gel electrophoresis

Answer 21

DNA moves toward anode.