Brainscape - Sheet1 Flashcards
A. Conjugation in Bacteria
In prokaryotes, the direct transfer of DNA between two cells that are temporarily joined. When the two cells are members of a different species, conjugation results in horizontal gene transfer.
B. Transduction
A process in which viruses carry bacterial DNA from one bacterial cell to another. When these two cells are members of a different species, transduction results in horizontal gene transfer.
C. Operon (inducible and repressible)
The operator, promoter, and the genes they control in a pathway. Inducible: presence of molecule activates operon. Repressible: presence of molecule inhibits operon.
D. Transformation
A change in genotype and phenotype due to the assimilation of external DNA by a cell. When the external DNA is from a member of a different species, transformation results in horizontal gene transfer.
E. Plasmid
A small, circular, double stranded DNA molecule that carriers accessory genes separate from those of a bacterial chromosome; in DNA cloning, used as vectors.
F. Polymerase Chain Reaction (PCR)
Any segment of DNA can be amplified in a test tube. DNA denatured to separate strands. Primers are added as DNA is annealed. Heat stable DNA polymerase extends the primers.
G. Gel Electrophoresis
Gel made of a polymer with electrical flow used to separate nucleic acid or proteins on the basis of size, electrical charge and other physical properties. DNA is negatively charges so they travel towards positive end. The gel separates the longer molecules.
H. DNA Ligase
A linking enzyme essential for DMA replication: catalyzes the covalent bonding of the 3’ end of DNA fragment to the 5’ end of another DNA fragment.
I. DNA Polymerase
An enzyme that catalyzes the elongation of new DNA (for example, at a replication fork) by the addition of nucleotides to the 3’ end of an existing chain. There are several different DNA polymerases; DNA polymerase III and DNA polymerase I play major roles in DNA replication in E. coli.
J. Okazaki Fragments
A short segment of DNA synthesized away from the replication fork on a template strand during DNA replication. Many such segments are joined together to make up the lagging strand of newly synthesized DNA
K. Leading vs Lagging Strand
Leading Strand: The new complementary DNA strand synthesized continuously along the template strand toward the replication fork in the mandatory 5’-3’ direction Lagging Strand: A discontinuously synthesized DNA strand that elongates by means of Okazaki fragments, each synthesized in a 5’-3’ direction away from the replication fork.
L. Topoisomerase
A protein that breaks, swivels, and rejoins DNA strands. During DNA replication, topoisomerase helps to relieve strain in the double helix ahead of the replication fork.
M. Primer
A strand of nucleic acid that serves as the starting point for DNA synthesis
N. Replication Fork
Structure that forms in DNA replication; created by helicases that create two separate DNA strands that serve as templates for leading and lagging strands
O. Helicase
Enzyme that unzips DNA helix for DNA synthesis
P. Restriction enzymes
enzyme that cuts DNA at or near specific recognition nucleotide sequences known as restriction sites
Glycosidic Linkage
a covalent bond formed between two monosaccharides by a dehydration reaction
Polysaccharides
polymers with a few thousand monosaccharides joined by glycosidic linkages
Polypeptides
amino acids connected by covalent peptide bond.
double helix
Structure of DNA: sugar-phosphate backbones on outside, nitrogenous base son inside, bases joined by hydrogen bonding
Endoplasmic reticulum
active in membrane synthesis and other synthetic/metabolic processes
Ribosomes
Make proteins, free in cytosol or bound to rough ER or nuclear envelope
Golgi apparatus
organelle active in synthesis, modification, sorting, and secretion of cell products
Lysosome (animal cell only)
digestive organelle where macromolecules are hydrolyzed
Mitochondrion
organelle where cellular respiration occurs and most ATP is generated
Peroxisome
organelle with various specialized metabolic functions; produces hydrogen peroxide as a by-product, then converts it to water
Cytoskeleton
reinforces cell’s shape; functions in cell movement, components made of protein. Includes microfilaments, intermediate filaments, microtubules
Centrosomes
regions where the cell’s microtubules are initiated; contains a pair of centrioles (centrioles only in animal cells)
Flagellum
(animal cell only): motility structure present in some animal cells, composed of a cluster of microtubules within an extension of the plasma membrane
Nuclear envelope
double membrane enclosing the nucleus, perforated by pores, continuous with ER
Nucleolus
nonmembranous structure involved in production of ribosomes; a nucleus has one or more nucleoli
Central vacuole
(plant cell only): functions include storage, breakdown of waste products, hydrolysis of macromolecules; enlargement of vacuole is a major mechanism of plant growth
Chloroplast
(plant cell only): photosynthetic organelle; converts light energy to chemical energy stored in sugar molecules
Plasmodesmata
(plant cell only): cytoplasmic channels through cell walls that connect the cytoplasm’s of adjacent cells
Cell wall
(plant cell only): outer layer that maintains cell’s shape and protects cell from mechanical damage; made of cellulose, other polysaccharides, and protein
Fluid mosaic model
membrane is fluid structure with various proteins embedded in or attached to the lipid bilayer.
Diffusion
Movement of molecules of any substance so that they spread out evenly in the available space
Facilitated diffusion
transport proteins help ions and hydrophilic substances cross the membrane.