Bunyavirus & Arenavirus Flashcards Preview

MCIM 309 > Bunyavirus & Arenavirus > Flashcards

Flashcards in Bunyavirus & Arenavirus Deck (15):
1

Bunyaviridae Group

SUPERGROUP! includes 200+ enveloped segmented ssRNA
most are arboviruses (arthropod)

2

Bunya Structure

spherical 90-120nm
envelope has 2 proteins G1 &G2
has 3 ssRNA

3

Bunya Pathogenesis

Viremia, secondary viremia, and target sites such as CNS, Liver, Kidney, vascular endothelium
Induce type 1 interferons

4

Bunya Symptoms

Usually mild nonspecific febrile, flulike, viremia related illness. fever ~ 3 days
Encephalitis illnesses
~1 week incubation, fever, headache, lethargy, vomiting,
Seizures, meningitis, <1% death.
Hemorrhagic fevers (rift valley fever)
Hantavirus: pulmonary syndrome. resp failure.

5

Bunya Diagnosis

RT-PCR on viral RNA
ELISA may detect antigen in clinical specimens with intense viremia

6

Bunya Control

no specific therapy.
kill mosquitos and rats(hantavirus)

7

Arenaviruses include:

Lymphocyte choriomeningitis(LCMV)
Hemorrhagic fever viruses: Lassa, Junin, Machupo.

8

Arena structure

enveloped virion, 2 circular ambi-ssRNA

9

Arena Pathogenesis

10-14 days
infect macrophages, induce cytokine + interferon. cell and vascular damage.
T cell immunopathies

10

Arena Epidemiology

Africa + South America
zoonosis via rodents
infect humans through: inhalation of aersols, contaminated food, fomites. (not usually bites)

11

Arena LCMV symptoms

Febrile + flulike myalgia
10% progress to CNS
perivascular mononuclear infiltrates

12

Arena Lassa and other hemorrhagic fevers symptoms

Fever(duh), coagulopathy, petechiae,
liver and spleen necrosis.
hemorrhage and shock.
occasional cardiac and liver damage.
death in 50%

13

Diagnosis

RT-PCR for viral genome.
too dangerous for isolation
level3 for LCM
level 4 for Lassa + others

14

Treatment

Ribavirin (of course)
supportive therapy (so nothing really)
don't touch rats.

15

Immune response

requires CMI but antibody can help viremic spread.