CPAC 4 - pollen tube growth

Question 1

method - pollen tube growth

Answer 1

1. make up 2cm of each sucrose concentration (0 - 40%).
2. label one petri dish per sucrose concentration and place a damp piece of filter paper in each.
3. collect 5 microscope slides and label each one. Add 2 drops sucrose and mineral salt medium to each.
4. collect a flower with several mature anthers and gently rub with a mounted needle to rub pollen onto slides.
5. place in petri dishes for 10 mins. Look at undr microscope and calculate average pollen tube length.