Cross Match

Question 1

Why do blood typing

Answer 1

Attempt to match recipient to a donor with similar

blood groups

Question 2

Why do a crossmatch

Answer 2

Incubate recipient/donor RBCs with recipient/donor

serum and look for reaction

Question 3

What does crossmatching do

Answer 3

○ Detects agglutinating antibodies

○ May detect hemolytic antibodies if complement added

Question 4

What are the 3 types of crossmatching

Answer 4

Major Crossmatch
Minor Crossmatch
Patient Autocontrol

Question 5

How to do a major crossmatch

Answer 5

Patient Serum + Donor RBC

Question 6

How to do a minor crossmatch

Answer 6

Donor Serum + Patient RBC

Question 7

How to do a patient autocontrol

Answer 7

Patient Serum + Patient RBC

Question 8

Explain how to do a crossmatch

Answer 8

Serum + RBC and incubate for 15 minutes and centrifuge

Question 9

What are the limitations of crossmatching

Answer 9

● Patient Autoagglutination
○ Voids Blood Typing Cards and Gel Column results
○ Voids Crossmatch results

Question 10

Cross matching may not detect antibodies causing what 3 reasons

Answer 10

○ Hemolysis (unless complement added)
○ Accelerated RBC destruction
○ Delayed onset transfusion reactions (ex. fever)

Question 11

What are the transfusion screening guidelines for a dog

Answer 11

○ DEA 1.1 blood typing
■ ‘Debatable’ for 1st time transfusion
■ DEA 1.1 (-) dogs get 1.1 (-) blood
■ DEA 1.1 (+) dogs get 1.1 (+) blood
○ Crossmatch
○ Universal Blood Donor:
■ DEA 1.1 (-), 1.2 (-), 3 (-), 5 (-), 7 (-), and 4 (+)

Question 12

What are the transfusion screening guidelines for a cat

Answer 12

○ ALWAYS Blood type and crossmatch
○ Type A cats get type A blood
○ Type B cats get type B blood
○ Type AB cats get either type A or B blood

Question 13

What are the transfusion screening guidelines for a species other than cat or dog

Answer 13

Crossmatch