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AUBF 1 Final > CSF PROTEIN DETERMINATION > Flashcards

CSF PROTEIN DETERMINATION Flashcards

1
Q

Preferred method for CSF Protein determination

A

Turbidimetric

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2
Q

T/F.
Turbidimetric precipitates only albumin

A

FALSE.
Turbidimetric precipitates both albumin and globulin

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3
Q

It precipitates albumin only:

A

sulfosalicylic acid

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4
Q

To precipitate globulins, add

A

sodium sulfate

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5
Q

Dye binding uses

A

Coomassie Brilliant Blue

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6
Q

assess the integrity of the blood brain barrier

A

CSF /Serum Albumin Index

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7
Q

CSF /Serum Albumin Index
Normal value:
Abnormal value:

A

Normal value: <9
Abnormal value: >9

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8
Q

Index of 100:

A

complete damage to BBB

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9
Q

assess condition with IgG production within the CNS (Multiple Sclerosis)

A

IgG Index

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10
Q

indicative of IgG production within the CNS

A

IgG index

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11
Q

IgG Index
Normal value:
Abnormal value:

A

Normal value: <0.70
Abnormal value: >0.70

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12
Q

Done in conjunction with serum electrophoresis

A

CSF electrophoresis

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13
Q

For the detection of oligoclonal bands

A

CSF electrophoresis

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14
Q

The presence of 2 or more oligoclonal bands in CSF but not in serum is valuable for the diagnosis of:

A

Multiple sclerosis
Neoplastic disorders
Encephalitis
Guillain-Barre’ syndrome

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15
Q

Protein component of the lipid-protein complex that insulate the nerve fibers

A

Myelin Basic Protein

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16
Q

presence of MBP in CSF indicates destruction of

A

myelin sheath

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17
Q

Used to monitor course of multiple sclerosis

A

Myelin Basic Protein

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18
Q

____ techniques are used for measurement

A

Immunoassay

19
Q

Done in conjunction with blood glucose.

A

CSF Glucose

20
Q

Specimen for blood glucose should be drawn ____ to spinal tap

A

2 hours prior

21
Q

CSF Glucose Normal values

A

60-70% of the plasma glucose

22
Q

CSF Glucose is increased

A

Due to increase plasma glucose (not
significant)

23
Q

CSF Glucose is decreased in

A

Bacterial, Fungal, and Tubercular Meningitis

24
Q

CSF glucose is normal in

A

Viral Meningitis

25
Q

CSF lactate levels can be a valuable aid in diagnosing and managing ____

A

meningitis

26
Q

CSF lactate normal values

A

Normal value: 10-22 mg/dl (1.1 to 2.4 mmol/L)

27
Q

T/F.
CSF Lactate is directly proportional to glucose

A

CSF lactate is inversely proportional to glucose

28
Q

Frequently CSF lactate levels are used to monitor patients with

A

severe head injuries

29
Q

RBCs contain high concentrations of lactate, and results that are falsely elevated may be obtained on

A

Xanthochromic or hemolyzed fluid

30
Q

CSF Lactate Values:
Bacterial meningitis
Fungal and tubercular meningitis
Viral meningitis

A

Bacterial meningitis - Increased (>35mg/dl)
Fungal and tubercular meningitis - Increased (>25mg/dl)
Viral meningitis - Normal (remains lower than 25mg/dl)

31
Q

Product of ammonia and alpha-ketoglutarate

A

CSF Glutamine

32
Q

Indirect test for the presence of excess ammonia in CSF

A

CSF Glutamine

33
Q

Why CSF Glutamine is preferred over direct measurement of CSF ammonia?

A

because the glutamine concentration remains more stable than the volatile

34
Q

CSF Glutamine Normal value

A

8 to 18 mg/dl

35
Q

CSF Glutamine is increased in

A

Reye’s syndrome, Disturbance of
consciousness/coma (more than 35 mg/dl)

36
Q

CSF Enzyme for Brain tissue LD

A

LD 1 and 2

37
Q

CSF Enzyme for Lymphocytes LD

A

LD 2 and 3

38
Q

CSF Enzyme for Neutrophils LD

A

LD 4 and 5

39
Q

Serum LDH
Normal =
Flipped pattern =

A

Normal = LDH 2>1>3>4>5
Flipped pattern = LD 1>2>3>4>5 (AMI)

40
Q

CSF LDH
Normal =
Neurological abnormalities=
Bacterial meningitis=

A

Normal = LDH 2>3>1>4>5
Neurological abnormalities= LDH 1>2>3>4>5
Bacterial meningitis= LDH 5>4>3>2>1

41
Q

Detects gram-negative bacterial endotoxin

A

Limulus Lysate Test

42
Q

Limulus Lysate Test Reagent

A

Blood of horseshoe crab

43
Q

Positive result for Limulus Lysate Test

A

Clumping or clot formation

AUBF 1 Final (14 decks)

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