DNA Flashcards
1
Q
what direcection do we sequence?
A
5’ to 3’
2
Q
what are the purines
A
adenine and guanine
3
Q
purines have:
A
2 carbon rings (smaller name, bigger structure)
4
Q
what are the pyrimidines
A
thymines and cytosine
5
Q
pyrimidines have
A
one carbons ring (big name, smaller structure)
6
Q
transition is
A
purine to purine
pyrimidine to pyrimidine
7
Q
transversion are
A
purine to pyrimidine
or
pyrimidine to purine
8
Q
what are snp’s
A
single nucleotide polymorphisms
9
Q
what is polymorphism?
A
Genetic variation
10
Q
whats this
A
single nucleotide polymorphism
11
Q
what can we use for dna analysis
A
tissue, blood, hair, feaces
12
Q
how do we analyse the dna
A
pcr amplification of the pargeted fragment
13
Q
how can we determine genotypes
A
pcr assay or targeted sequencing
14
Q
PCR advantages
A
- use for small tissue samples
- can use crude dna extraction
- can determine sequence
15
Q
sushi wale story
A
- went to tokyo hotel, ordered sushi and extracted DNA from wale meat, found out they were selling illegal wales.
