Enterococcus Media and Biochemicals

Question 1

TSI yellow on bottom

Answer 1

Ferment glucose

Question 2

TSI yellow on top

Answer 2

Can utilize lactose/sucrose

Question 3

TSI red on top

Answer 3

Cannot use lactose/sucrose
Uses peptides

Question 4

TSI red on bottom

Answer 4

Can’t use glucose

Question 5

TSI black on bottom

Answer 5

Converts thiosulfate into H2S

Question 6

lactose fermentors (3)

Answer 6

E. coli
Klebsiella
Enterobacter

Question 7

Sucrose fermentors (4)

Answer 7

Serratia
Yersinia
P. vulgaris
P. mirabilis

Question 8

Indole test reagents

Answer 8

Para-dimethylaminobenzaldehyde
Kovac or Ehrlich’s reagent

Question 9

Indole test reactions

Answer 9

Tryptophan -> Indole + aldehyde -> colored complex

Question 10

SIM agar tests for (3)

Answer 10

H2S
indole
motility

Question 11

MIO tests for (3)

Answer 11

motility
ornithine decarboxylase
indole

Question 12

Ornithine decarboxylase pos color

Answer 12

pink

Question 13

Indole pos color

Answer 13

red

Question 14

Ornithine decarboxylase pos MOs (2)

Answer 14

K. aerogenes
E. cloacae

Question 15

Indole pos MOs (3)

Answer 15

E. coli
K. oxytoca
P. vulgaris

Question 16

MR/VP broth tells you

Answer 16

pathway used to produce pyruvate from glucose fermentation

Question 17

Klebsiella can use ____(2)____ as a sole source of carbon

Answer 17

Sodium Citrate

Question 18

IMViC characterization is for:

Answer 18

Indole
Methyl red
Voges-Proskauer
Citrate utilization

Question 19

E. coli IMVC

Answer 19

Indole: pos
Methyl red: pos
Voges-Proskauer: neg
Citrate utilization: neg

Question 20

Klebsiella Pneumoniae IMVC

Answer 20

Indole: neg
Methyl red: neg
Voges-Proskauer: pos
Citrate utilization: pos

Question 21

Enterobacter cloacae IMVC

Answer 21

Indole: neg
Methyl red: neg
Voges-Proskauer: pos
Citrate utilization: pos

Question 22

Citrobacter freundii IMVC

Answer 22

Indole: neg
Methyl red: pos
Voges-Proskauer: neg
Citrate utilization: pos

Question 23

Decarboxylation and deamination produce ____ products

Answer 23

alkaline

Question 24

phenylalanine deaminase (PDA) testing determines if MO can

Answer 24

deaminate

Question 25

Deamination is an _____ process

Answer 25

aerobic

Question 26

Decarboxylation is an _____ process

Answer 26

anaerobic

Question 27

Lysine Iron Agar LIA determines (3)

Answer 27

Deaminate lysine
Decarboxylate lysine
H2S production

Question 28

Proteus, Providencia, and Morganella are the only Enterobacteriaceae that ______(2)

Answer 28

deaminate lysine

Question 29

LIA interpretation purple/yellow

Answer 29

K/A = glucose fermentation

Question 30

LIA interpretation purple/purple

Answer 30

K/K = lysine decarboxylation

Question 31

LIA interpretation red/yellow

Answer 31

R/A = lysine deamination and glucose fermentation

Question 32

Methyl red fermenters (3)

Answer 32

E. coli
Citrobacter
Serratia

Question 33

indole negative

Answer 33

K. pneumoniae
P. mirabilis
Salmonella

Question 34

Motility negative

Answer 34

Klebsiella
Shigella

Question 35

H2S producers

Answer 35

Proteus
Edwardsiella
Salmonella

Question 36

Salmonella/shigella agar contains (3)

Answer 36

pH indicator
Lactose
H2S indicator

Question 37

Hektoen enteric agar contains (3)

Answer 37

H2S production
Lactose/sucrose/salicin fermentation
pH indicator

Question 38

Xylose lysine desoxycholate (xld) agar (3)

Answer 38

Lactose/sucrose/xylose
Phenol red
H2S production

Question 39

Orthonitrophenyl-beta-d-galactopyranoside (onpg) test detects

Answer 39

Beta-galactosidase and true fermenters

Question 40

Kligler’s Iron Agar (KIA) detects

Answer 40

Glucose/lactose usage
Protein use
H2S production

Question 41

VP detects

Answer 41

acetoin

Question 42

MR detects

Answer 42

acid

Question 43

citrate utilization positive

Answer 43

blue

Question 43

citrate utilization positive

Answer 43

blue

Question 44

Salmonella IMVC

Answer 44

• • • +

Question 45

Hafnia has a delayed _______ rxn

Answer 45

Citrate