Enzymes experiment (B1 to 3)

Question 1

How to find the optimum conditions for amylase to digest starch?

Answer 1

1. Add iodine solution into each dimple of spotting tile
2. Add starch solution and amylase to boiling tube.
3. Remove mixture from boiling tube every 10 seconds and add to spotting tile.
4. When amylase has fully digested starch, no colour change will be observed.

Question 2

What’s water bath used for?

Answer 2

Water bath is used to heat solutions

Question 3

Benefits of using water bath?

Answer 3

Keep solution at constant temperature throughout the experiment.