Exam 2 Flashcards
What is photorespiration?
when Rubisco fixes O2 (oxygenase activity) instead of CO2. This lowers the carbon count and “undoes” photosynthesis. This makes the plant less efficient
What are the effects in plants due to photorespiration?
One of the molecules produced from the addition of O2 to RuBP is processed in sections that consume ATP and release CO2 in order to regenerate 3PGA. Part of the pathway occurs in the chloroplasts and part of it occurs in peroxisome/mitochondria. Reaction sequence resembles respiration b/c it consumes O2 and produces CO2. It undoes photosynthesis b/c it consumes energy and releases fixed CO2—>decreases rate of photosynthesis
It can be protective for when plants are under high light and low CO2 conditions
Why might RUBISCO have both carboxylase and oxygenase activity?
O2 was the result of the creation of Rubisco
Carboxylase activity fixes CO2 (CO2+RuBP a 2PGA (3 carbons each)) —leads to sugar/starch synthesis w/new carbon
Oxygenase activity binds with oxygen (photorespiration)
O2 + RuBP a PGA (3 carbons) + PG (2 carbons)
—no new carbon gained, energy is consumed and CO2 released
Why might RUBISCO have both carboxylase and oxygenase activity?
Carboxylase activity is efficient in the current environment b/c there is a high concentration of CO2 and plants need CO2 in order to fix carbon to create energy under photosynthesis. Oxygenase activity is included b/c RUBISCO evolved under conditions in which the environment didn’t have oxygen or very little of it. Rubisco’s active sites are limited, the active site for O2 and the active site for CO2 seem to be connected, if you change one, you change the other
What is a C3 plant?
C3 plants fix CO2 in Rubisco to make a 3 carbon PGA in the bundle sheath fibers
C3 photosynthesis is the normal photosynthesis
C3 photosynthesis occurs in mesophyll cells on surface of leaves
What is a C4 plant?
C4 plants use PEP carboxylase to fix CO2 to make a 4 carbon compound in the mesophyll
C4 photosynthesis occurs in a different space than C3
C4 photosynthesis also occurs in the mesophyll cells but the 4-carbon compound that is produced is then transferred to the bundle sheath cells; consumes energy
C4 plants have Kranz anatomy—thicker bundle sheath cells due to increase in concentration of chloroplast in that organelle
enzyme that fixes CO2 into its 4-carbon compound is PEP carboxylase (PEPC)
What is the function of Kranz anatomy is C4 leaves?
has thicker bundle sheath cells in C4 leaves due to increased concentration of chloroplast.
4-carbon compound is transferred from the mesophyll leaf cells to the bundle sheath cells to concentrate the amount of CO2. In this way, there is no loss of CO2 due to photorespiration and plants would need less Rubisco for photosynthesis
What are the benefits of C4 photosynthesis?
It acts as a CO2 concentrator, it allows for the stomata to be open less which results in less water loss
There is a higher concentration of CO2 in the bundle sheath cells which means that there will be a decreased change in O2 binding to the active site and setting off photorespiration.
B/c there is a high concentration of CO2, plants need less amounts of Rubsico for photosynthesis
In C4 photosynthesis, CO2 is fixed more efficiently by PEPC which allows for stomata to be open less and this conserves water
What are the costs of C4 photosynthesis?
It is more energy costly
It is also only more advantageous in environments that are hot and dry
Uses more ATP
Where would you expect to find C4 plants?
In hot and dry environments
Found in conditions of drought, high temperatures, and nitrogen or CO2 limitation
In measuring photosynthesis in crop species you find that two species respond differently to a decrease in the concentration of O2 in the gas surrounding the leaf. One species (Species A) maintains a constant rate of photosynthesis in both high and low O2 concentration while the other exhibits a significant increase in photosynthesis in the low O2 environment. Propose hypothesis to explain observation
Hypothesize that Species B is a C4 plant since its photosynthetic rate increases in a low O2 environment. PEPC doesn’t perform well under conditions of high concentrations of O2
Compare and contrast C4 and CAM photosynthesis by following a molecule of CO2 from initial fixation from the atmosphere to synthesis of sugar following the Calvin-Benson cycle
Same in C4 and CAM: initial CO2 fixation is with PEP carboxylase
Difference in C4 and CAM: release of CO2 for fixation with Rubisco is at a different time
Rubisco and PEPC reactions are separated in time
For a C4 plant: these reactions are separated by location
In CAM they are separated by time
CAM plants
Stomata is only open at night
It can fix CO2 with PEP carboxylase at night in the mesophyll and then perform the calvin cycle during the day when there is solar energy to power it
C4 plants
The binding to PEP carboxylase can happen at the same time but it occurs in the bundle sheath rather than the mesophyll
Describe the cost of mycorrhizal associations for plants.
They have to maintain bacteria in their cells
Costs to the plant are often a percentage of their carbon, b/c they have to supply mycorrhizae with carbon and energy