Exam 2

Question 1

Which of the following reactions under standard condition is not highly exergonic ( ∆G’ᵒ ≤ - 10 KJ/mol )

Answer 1

the reaction catalyzes for succinyl CoA synthetase.

Question 2

In the breakdown of nucleotide in the brain, amino group which is produced will

Answer 2

Be transported to the liver in form of Glutamine

Question 3

When α-ketoglutrate is metabolized to oxaloacetate via citric acid cycle, how many ATP equivalents are formed per molecule of α-ketoglutrate.

Answer 3

7.5 ATP formed per molecule of α-ketoglutrate

Question 4

To conversion of one mole of pyruvate to 3 mol of CO2 via pyruvate dehydrogenase and the CAC also yield _____ mol of NADH, _____ mol of FADH2 and ____mol of ATP(GTP)

Answer 4

4, 1, 1

Question 5

Which cofactor not correctly matched with a group that it helps to transfer from one

Answer 5

Lipoate: -CO2

Question 6

Anaplerotic reaction:

Answer 6

Produce oxaloacetate and malate to maintain constant level of citric acid cycle intermediate.

Question 7

180 Ib male receive 2500 Kcal/d how many grams of protein are needed?

Answer 7

Range depends on 10-35% Kcal/d of protein

Question 8

Which of the following statement is true:

Answer 8

Elevated level of NADH decrease the activity of citrate synthase.

Question 9

Clear sign of phenylketoneurea is:

Answer 9

Phenylalanine in urine

Question 10

Which enzyme or enzyme complex with arsenite block.

Answer 10

α-ketoglutrate dehydrogenase complex

Question 11

Decrease in the level of ……. and ……. lead to decrease in the synthesis of DNA

Answer 11

Folate and Vit B12

Question 12

Which of the following statement is true:

Answer 12

ATP activate a kinase which inactivate pyruvate dehydrogenase

Question 13

Urea cycle:

Answer 13

Requires 1.5 ATP equivalent

Question 14

Tetrahydrobiopterin is used in the oxidation of

Answer 14

Phenylalanine

Question 15

The oxidative decarboxylation of α-ketoglutrate proceeds by means of multi-step reactions in which all but one of the following factors are required. Which one is not required?

Answer 15

Biotin

Question 16

FAD occur in

Answer 16

Succinate dehydrogenase

Question 17

Which one of the following enzymatic activities would be ……(missing part)

Answer 17

α-ketoglutrate dehydrogenase complex

Question 18

Which of the following Vitamins is synthesized by bacterial GI:

Answer 18

Vit B12

Question 19

Vitamin Niacin is synthesized from:

Answer 19

Tryptophan

Question 20

Table question about PDCAAS (slide No. 53 in AA oxidation handout)

Answer 20

Something in 50’s range (maybe 51) - answer is A

Question 21

CH3 C12COH (not sure from the molecule) the molecules enter the B-oxidation and citric acid cycle:

1. How many ATP equivalent required for this reaction:
2. Moles of NADH formed:
3. Molecules of FADH2 formed:

Answer 21

1. 2 ATP
2. 6 + (7*3) = 27 ATP
3. 6 + (7*1) = 13 ATP

Question 22

Which of the following is not true about regulation of pyruvate dehydrogenase complex:

Answer 22

Elevated level of NADH increase the activity of the complex.

Question 23

Which combination of cofactors is involved in conversion of pyruvate to acetyle CoA.

Answer 23

FAD, TPP and lipoic acid

Question 24

Protein serum albumin:

Answer 24

Carry fatty acid from adipose tissue to organ.

Question 25

Reaction of citric acid cycle that produce an ATP equivalent:

Answer 25

Succinyl coA to succinate

Question 26

Controls amino acid catabolism:

Answer 26

Carbamoyl phosphate

Question 27

Investigators at MCPHS have recently discovered a compound they have dubbed CACarin. If this compound is added to a mitochondrial preparation that is oxidizing pyruvate as a substrate, the concentration of acetyl-CoA begins to increase. Which of the following enzyme does this compound most likely inhibit?

Answer 27

citrate synthase

Question 28

The following reaction ∆G= 29.7

L-malate →oxaloacetate

Answer 28

Can occur in a cell as a result of coupling to reaction catalyze by synthase.