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3S: Biochem-Lab > Exp 6.4: Lipids (Quali Tests) > Flashcards

Exp 6.4: Lipids (Quali Tests) Flashcards

1
Q

Hydroxamic Test:
purpose

A

Detect presence of carboxylic acid ester (glycerophospholipids, TAGs, cholesteryl esters)

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2
Q

Hydroxamic Test:
reagents

A

ethanol:butanol(3:1)
NH2OH x HCl
NaOH
FeCl3
HCl

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3
Q

Hydroxamic Test:
positive result

A

Burgundy color

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4
Q

Hydroxamic Test:
principle

A

Complexation Reaction

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5
Q

Acrolein Test:
purpose

A

Detect presence of glycerol (glycerophospholipids, TAGs,)

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6
Q

Acrolein Test:
positive result

A

Burnt fat odor

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6
Q

Acrolein Test:
reagents

A

KHSO4
water bath

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7
Q

Acrolein Test:
principle

A

Dehydration and oxidation

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8
Q

Libermann-Burchard Test:
purpose

A

Detect presence of unsaturated sterols (cholesterol)

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9
Q

Libermann-Burchard Test:
reagents

A

Conc. H2SO4
acetic anhydride

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10
Q

Libermann-Burchard Test:
positive result

A

Emerald green solution

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11
Q

Libermann-Burchard Test:
principle

A

Esterification of OH at C- 3 with acetic anhydride
Epimerization of double bond at C-5

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12
Q

Kraut’s Test:
purpose

A

Detect presence of choline (phosphatidyl choline, sphingolipids)

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13
Q

Kraut’s Test:
reagents

A

Bismuth nitraye
3M HNO3
KI (Kraut’s reagent)

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14
Q

Kraut’s Test:
positive result

A

Dark orange to red ppt

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15
Q

Kraut’s Test:
principle

A

Complexation reaction
● proposed condensation, dehydration & epimerization
● modification of Dragendorff’s test

16
Q

Ascorbic Acid Method:
purpose

A

Detect presence of phosphate (phospholipids)

17
Q

Ascorbic Acid Method:
reagents

A

6M HNO3
6M NaOH
Molybdate Rgt
0.1M Ascorbic Acid Solution

18
Q

Ascorbic Acid Method:
positive result

A

reduced to intensely colored molybdenum blue by ascorbic acid

19
Q

Ascorbic Acid Method:
principle

A

Ammonium molybbdate + potassium antimonyl tartrate react in acid medium with orthophosphate = heteropoly acid to phosphomolybdic acid

20
Q

Ninhydrin Test:
purpose

A

Detect presence of α-amino group

21
Q

Ninhydrin Test:
reagents

A

Triketohydrindene hydrate (ninhydrin in EtOH)

22
Q

Ninhydrin Test:
positive result

A

Blue-violet solution

23
Q

Ninhydrin Test:
principle

A

Oxidative deamination followed by condensation

24
Q

Test for Lipid Unsaturation:
purpose

A

Detect presence of double bonds; degree of unsaturation

25
Q

Test for Lipid Unsaturation:
reagents

A

5% Bromine / Iodine solution

26
Q

Test for Lipid Unsaturation:
positive result

A

Reddish brown
Violet coloration

27
Q

Test for Lipid Unsaturation:
principle

A

Addition reaction

28
Q

Molisch Test:
purpose

A

Detect presence of sugar moiety

29
Q

Molisch Test:
reagents

A

1% ethanolic α- napthol, conc. H2SO4

30
Q

Molisch Test:
positive result

A

Violet ring at the interface

31
Q

Molisch Test:
principle

A

Hydrolysis, dehydration, and condensation reaction

3S: Biochem-Lab (10 decks)

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