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Kaplan: Pathophysiology 1-5 > Fundamentals of Pathology > Flashcards

Fundamentals of Pathology Flashcards

1
Q

Hematoxylin typically has what color upon staining?

A

blue to purple color

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2
Q

Eosin typically stains what color?

A

pink to red

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3
Q

Hematoxylin typically stains what structures?

A

nucleic acids and Ca2+ salts as well as bacteria

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4
Q

Eosin typically stains what structures?

A

proteins (eg. thyroid colloid, cytoplasm, collagen , RBCs, fibrin)

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5
Q

Difference between histochemical and immunohistochemical stains.

A

histochemical stains involve chemical reactions whereas immunohistochemical stains involve tagging an ab with a stain/color

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6
Q

Name some histochemical stains. (Name at least 3)

A

Prussian blue, Congo red, Ziehl-Neelsen, PAS, gram stain, trichrome, reticulin

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7
Q

What does Prussian blue stain?

A

iron

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8
Q

What does Congo red stain?

A

amyloid

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9
Q

What does Ziehl-Neelsen stain?

A

acid-fast bacilli

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10
Q

What does periodic acid Schiff stain?

A

PAS stains high carbohydrate content molecules

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11
Q

What does Gram stain, stain for?

A

bacteria

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12
Q

What does trichome stain, stain for?

A

stains cells and connective tissue

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13
Q

What does reticulin stain for?

A

stain collagen type III molecules

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14
Q

Name some immunohistochemcial (antibody) stains. (name 3)

A

cytokeratin, vimentin, desmin, prostatę specific antigen PSA

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15
Q

What does cytokeratin stain for?

A

epithelial cells

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16
Q

What does vimentin stain for?

A

cells of mesenchymal origin (except the 3 muscle types; stains many sarcomas)

17
Q

What does desmin stain for?

A

the 3 muscle types

18
Q

What is IFM (immunofluorescence microscopy) typically reserved for?

A

renal and autoimmune disease

19
Q

What is transmission electron microscopy typically reserved for? (Name 2 things)

A

renal disease, neoplasms, infections, genetic disorders

20
Q

Molecular techniques that can be done to understand pathology?

A

protein electrophoresis, Southern and Western blots, PCR, cytogenetic analysis (karyotyping in situ hybridization studies)

Kaplan: Pathophysiology 1-5 (6 decks)

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