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FA Micro > General > Flashcards

General Flashcards

1
Q

bugs that don’t gram stain well

A 
These Microbes May Lack Real Color
Treponema
Mycobacteria
Mycoplasma
Legionella
Rickettsia
Chlamydia
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2
Q

why Treponema doesn’t gram stain well

A

too thin to be visualized

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3
Q

how to visualize treponema

A

dark field microscopy and fluorescent antibody staining

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4
Q

why mycobacteria doesn’t gram stain well

A

high lipid content in cell wall

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5
Q

how to visualize mycobacteria

A

carbolfuchsin in acid fast stain

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6
Q

why mycoplasma doesn’t gram stain well

A

no cell wall

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7
Q

why legionella pneumophila doesn’t gram stain well

A

primarily intracellular

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8
Q

how to visualize legionella pneumophila

A

silver stain

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9
Q

why rickettsia doesn’t gram stain well

A

intracellular

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10
Q

why chlamydia doesn’t gram stain well

A

intracellular

lacks muramic acid in cell wall

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11
Q

what stains with Giemsa stain

A

Certain Bugs Really Try my Patience

Chlamydia
Borrelia
Rickettsia
Trypanosomes
Plasmodium
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12
Q

PAS stain

A

stains glycogen and mucopolysaccharides

used to diagnose Whipple disease (Tropheryma whipplei)

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13
Q

use what stain to diagnose Whipple disease (Tropheryma whipplei)

A

PAS stain

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14
Q

Ziehl-Neelsen / carbol fuchsin stain is for

A

acid fast organisms

  • nocardia
  • mycobacterium
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15
Q

India ink stains

A

Cryptococcus neoformans - can also use mucicarmine to stain thick polysaccharide capsule red

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16
Q

Silver stain

A

Fungi: pneumocystis

Legionella

Helicobacter pylori

17
Q

special culture for H influenzae

A

chocolate agar w/ factors V (NAD+) and X (hematin)

18
Q

special culture for N gonorrhea and N meningitidis

A

Thayer Martin or VPN media

  • Vancomycin (inhibits gram pos)
  • Polymyxin (inhibits gram neg except Neisseria)
  • Nystatin (inhibits fungi)
19
Q

special culture for B pertussis

A

Bordet-Gengou (potato) agar

20
Q

special culture for C. diphtheriae

A

Tellurite agar

Loffler medium

21
Q

special culture for M. tuberculosis

A

Lowenstein-Jensen agar

22
Q

special culture for M. pneumoniae

A

Eaton agar, requires cholesterol

23
Q

special culture for lactose fermenting enterics

A

pink colonies on MacConkey agar (fermentation produces acid, turning colony pink)

24
Q

special culture for E. coli

A

eosin-methylene blue (EMB) agar - as colonies w/ green metallic sheen

25
Q

special culture for Legionella

A

charcoal yeast extract agar buffered w/ cysteine and iron

26
Q

special culture for Fungi

A

Sabouraud agar

FA Micro (1 decks)

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