Flashcards in Histopathology and cytopathology Deck (12):
What are the situations in which cytopathology might be used as a diagnostic method?
What are the main steps involved in processing a specimen?
Fix in formalin
Embed in paraffin wax
Rehydrated and stained with eosin and haematoxylin
What are the types of specimen that may be tested in histopathology ?
Biopsies 2-3 days
Resection specimens 5-7 days
In what situations would frozen sections be required?
During surgery of removal of cancer to make sure all of it has been removed
What are the advantages of frozen section diagnosis?
takes 30 minutes
What are the benefits of an autopsy?
Performed to find the cause of death
why treatment was unsuccessful
spread of disease
education on disease processes
What are the benefits of cytology screening?
Cervical and breast cancer screening
What are the types of specimen that may be tested in cytopathology?
Fine needle aspirates (less invasive than biopsies)
What is immunohistochemistry?
identify specific antigens using antibodies
What does the eosin stain do?
Stain cytoplasm pink
What does the haemotoxylin stain do?
Stain nuclei blue