Impregnation

Question 1

Ideal volume

Answer 1

25x

Question 2

3 ways of paraffin wax impregnagion

Answer 2

1) Manual
2) Automatic
3) Vacuum

Question 3

Requires 4 changes with 15 mins interval

Answer 3

Manual processing

Question 4

Makes use of an automatic tissue processing machine resulting in a more rapid diagnosis with less technicality

Answer 4

Automatic processing

Question 5

Impregnation under negative pressure; for urgent biopsies and delixate tissues; most rapid

Answer 5

Vacuum

Question 6

Simplest; most common; best for routine use; mp = 54-58 degC

Answer 6

Paraffin wax

Question 7

Mixture of highly purified paraffin wax and synthetic plastic polymers; more elastic and more resilient; mp: 56-57 degC

Answer 7

Paraplast

Question 8

Similar to paraplast but less brittle and less compressible; mp: 56-58 degC

Answer 8

Embeddol

Question 9

Semi-synthetic wax recommended for embedding eyes

Answer 9

Bioloid

Question 10

Harder than paraffin; not soluble in water but soluble in 94% ethanol and other clearjng agents; mo: 46-48 degC

Answer 10

Ester wax

Question 11

Mostly polyethylene glycols with mp of 38-42 degC or 45-56 degC

Answer 11

Water soluble waxes

Question 12

Most commonly used polyethylene glycol which appears solid at RT; soluble in water, hemce does not require dehydration and clearing; for enzyme histochemical studies

Answer 12

Carbowax

Question 13

Purified form of nitrolcellulose; soluble in many solvents; for specimens containg large cavities or hollow spaces; for hard and dense tossues; for large sections of whole embryos; less shrinkage and distortion

Answer 13

Celloidin

Question 14

Recommended for bones, teeth, large brain sections and whole organs; must be cut wet (both knife and tissue are kept moist with 70% alcohol)

Answer 14

Wet celloidin method

Question 15

For processing of whole eye sections; can be cut without alcohol due to presence of cedarwood oil

Answer 15

Dry celloidin method

Question 16

Mixture used in dry celloidin method; equal parts of chloroform and cedarwood oil

Answer 16

Gilson’s mixture

Question 17

Another form of celloidin that is soluble in equal concentration of ether and alcohol

Answer 17

Low viscosity nitrocellulose

Question 18

Used when dehydration is to be avoided; used for histochemical and enzyme studies; for delicate specimens and frozen sections; does not require dehydration and clearing

Answer 18

Gelatin impregnation