intro to vet diagnostic laboratories Flashcards

1
Q

lesion

A

a pathologic change in a tissue/cell

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2
Q

cause/etiology

A

casuative agen

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3
Q

pathogenesis

A

sequence of events that lead to or underlie a disease

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4
Q

prognosis

A

prediction of outcome of the pathologic process

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5
Q

diagnosis

A

a concise statement of the nature, cause of name of a disease

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6
Q

what is a biopsy

A

removal and examination of tussue from a live patient

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7
Q

why do a biopsy

A
  1. make or confirm a diagnosis
  2. rule out other diseases
  3. determine adequacy of excision
  4. provide prognostic information
  5. guide therapeutic decisions
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8
Q

the tissue is examined out of context, so interpretation relies:

A

on the clinical data you provide

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9
Q

what should a submission form always include

A
  • decription of lesion
  • location sampled
  • number of containers/specimens
  • signalment of patient
  • history/clinical data pertinent to case
  • specific questions to be answered
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10
Q

what are the most common types of biopsies

A
  1. endoscopic (pinch)
  2. needle core (Tru-Cut)
  3. punch
  4. incisional
  5. excisional
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11
Q

what is an endoscopic biopsy

A
  • small superficial tissue samples obtained thorugh and endoscope
  • usually respiratory, alimentary, or urogenital mucosa
  • multiple samples increase likelihood of a diagnosis
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12
Q

what is a needle core biopsy

A
  • long thin cylinders of tissue often obtained w/ ultrasound guidance
  • solid/parenchymatous tissues
  • multiple samples
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13
Q

what is a punch biopsy

A
  • multiple punch sizes available; bigger is better
  • avoid using tissue cassettes unless samples are very small
  • multiple/larger samples
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14
Q

what is incisional biopsy

A
  • wedge of tissue removed from a larger lesion or organ
  • avoid bloody or necrotic areas
  • larger tissue samples
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15
Q

excisional biopsy

A
  • usually elliptical excision
  • surgical margins can be identified & evaluated to determine adequacy of excision
  • submission of entire lesion
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16
Q

routine histochemistry

A

hemetoxylin & eosin (H&E)

17
Q

special histochemistry

A

highlights specific features that are poorly discerned with H&E

18
Q

immunohistochemistry

A

antibody-based detection of protein antigens in tissue sections

19
Q

hematoxylin

A

basic dye that binds negatively charged substances (nuclei, ribosomes)

20
Q

eosin

A

acidic dye that binds positively charged substances (cytoplasm, collagen, RBC)

21
Q

immunohistochemistry (IHC)

target antigens

A
  • cell structural components
  • enzymes & hormones
  • extracellular matrix components
  • infectious agents
  • cytokines & receptors
22
Q

samples for ICH should be formalin-fixed, but ….

what happens when prolonged?

A

prolonged fixation can have adverse effects

23
Q

what is an autopsy (necropsy)

A

a postmortem examination to discover the cause of death or extent of disease

24
Q

why do an autopsy

A
  1. diagnostic (cause, heard health, zoonotic, closure)
  2. research
  3. teaching
  4. insurance requirement
  5. forensic
25
Q

what does accurate diagnosis rely on

A
  • clinical history and accompanying clinical data
  • postmortem condition
  • correct lesion identification and sampling
  • accurate results of additional testing
26
Q

final diagnosis

A

integration of all findings to provide a reasonable pathophysiologic explanation for disease/death