IS PART 2 - Immunoglobulins Flashcards
(28 cards)
glycoprot found in the serum portion of blood
immunoglo
ig are composed of
82-96% polypep
2-14% carbs
ig migrates at the gamma reg when subj to ph
8.6
major humoral component of adaptive iR
ig
intrachain and interchain bonds
disulfide bonds
flexible reg of ig: allows for movement of the fab arms
hinge reg
hinge region is rich in
located between
proline
ch1 and ch2
domains
1 LC =
1 hc =
lc = vl + cl hc = vh + 3ch Ig'GAE'
isotype
allotype
idiotype
heavy chain det ig class constant reg variable reg
basic ig structure
monomer
2h + 2lc
ig GDE
2 monomers with secretory component and j chain
dimer - igA
pentamer
IgM polymer (crab, star)
fragment igm by 2
2-mercaptoethanol (used in bb)
dithiotreitol (dtt)
fragment monomer by 2
papain
pepsin
cleaves above hinge; 3 fragments… 2fab 1fc
below ; 2 frag …2fab
papain
pepsin
fxn of fc
biologic act
comple fixa
skin fixa
placental trans
major ig in adults
igG
maj Ab in secondary IR
IgG
IgG1
2
3
4
66% = 2 disfb 23 = 4 disfb 7 = 15 4 = 2
Ig crosses placenta
most effective
least
igG1
igG2
fixation complement
most effective
least
igG3
igG4
best in agglu
best in precip
igM
igG
most efficient in complement fixa and agglu
igM
igA1
igA2
serum = monomer secretions = dimer
