Kool Aid Experiment Flashcards
How does Column Chromatograhpy seperate its components?
Based on their polarities (ie. polar on the bottom, non-polar at the top)
Stationary phase
A solid compound that fills the column and interacts with the mobile phase
Mobile Phase
Solvent in which the mixture of compounds is dissolved
Dipole moment
A measurement of the separation of a charge in a molecule
Difference between the stationary phase and the mobile phase
different polarity
What causes dipole moments?
an uneven distribution of electron density
Why does the speed (flow) change in the column?
due to the different polarities it will depend on how strongly they interact with the stationary phase
What does “like attracts like” mean?
The more polar component of the mixture will interact with the polar phase, and the less polar component with the less polar phase
What happens to the component that interacts strongly with the stationary phase?
it will be held in the column tighter, which means it flows slowly.
What happens to the component that interacts strongly with the mobile phase?
flows at a faster rate, exits column first
In the Kool Aid experiment, what was the mobile phase and stationary phase?
mobile - deionized water (dissolved the Kool Aid)
stationary - sand
Is silica more polar than water or not?
more
Spectra
a chart or a graph that shows the intensity of light (wavelengths) being emitted of a certain molecular system
Abosrbance
A measurement of the ability of a molecule to absorb light of a specific wavelength
Transmittance
Measurement of how much light passes through a substance (ratio: light out = light in)
