L6 Protein expression

Question 1

Trans acting factor

Answer 1

The regulatory protein that binds to a cis-acting sequence

Question 2

A regulatory protein (_______) binds to a particular seq of DNA (____)

Answer 2

trans acting factor, cis

Question 3

Operon

Answer 3

unit of bacterial gene expression and regulation

Question 4

LacZ gene is the ___ gene in the operon. IT encodes ____, which breaks down

Answer 4

first, B-galactosidase, which breaks down lactose into galactose and glucose

Question 5

In the absence of lactose, the Lac repressor binds the ___ and

Answer 5

operator and excludes RNA polymerase

Question 6

In the presence of lactose

Answer 6

Lac repressor dissociates from the DNA

Question 7

CAP recruits ___ and binds to the CAP site

Answer 7

RNA pol

Question 8

Transcription is ____ in the absence of lactose with or without glucose

Answer 8

respressed

Question 9

In Lac operon regulation, what happens when E.coli is grown in medium containing both glucose and lactose

Answer 9

Neither CAP nor the lac repressor are bound to the DNA

Question 10

How does the CAP work in the lac operon regulation?

Answer 10

Cyclic AMP binds to CAP, which then binds its CAP site, and activates transcription