Lab 1

Question 1

What is the objective of the air exposure slide?

Answer 1

to determine if their are culturable microbes in the air

Question 2

what is a medium?

Answer 2

a liquid gel designed to support bacteria/cell growth

Question 3

A liquid medium is?

Answer 3

broth

Question 4

A gel medium is?

Answer 4

agar

Question 5

Define agar

Answer 5

chemical derived from seaweed that solidifies into a jellylike semisolid

Question 6

What are the types?

Answer 6

Plate and a slant

Question 7

__________=general medium

Answer 7

typical soy agar

Question 8

What is the objective of a surface swab?

Answer 8

to determine if their are culturable microbes on my phone

Question 9

Define sterilization

Answer 9

kills all microbial containments

Question 10

What is an example of sterilization?

Answer 10

Autoclaving

Question 11

Define disinfection and give example?

Answer 11

Kills most microbes and an example is lysol

Question 12

Why do you work near bunsen burner when streaking plates?

Answer 12

to create a vacuum near the plate so microbes in the air dont contaminate the loop

Question 13

What is the objective to Aseptic technique and transfering cultures?

Answer 13

to subculture staohlocoocus aneus and to demonstrate proper aseptic technique

Question 14

Define aseptic techique

Answer 14

Any technique done to prevent contamination when working with microbes

Question 15

Define subculture

Answer 15

transfer a microbe from one medium to another

Question 16

Define pure culture

Answer 16

Culture of bacteria containing only 1 strain

Question 17

aerobic bacteria need what?

Answer 17

o2

Question 18

Where is Bacterium S. aureus found?

Answer 18

in the human respiratory tract and on human skin

Question 19

What can S aureus cause?

Answer 19

Food poisoning
Skin infections
respiratory disease
staph infection

Question 20

What is the objective of a 4 way streak?

Answer 20

to learn important techniques which dilute the bacteria into single, discrete, colonies

Question 21

What does bacteria Micrococcus luteus found?

Answer 21

Normal flora on human skin