Lab

Question 0

Ampicillin is the _____ for gram negative bacteria

Answer 0

Antibiotic

Question 1

Drug resistance is the ______ or metabolic advantage that the plasmid gives to bacteria

Answer 1

Selectable marker

Question 2

Gfp glowing gene is activated by the presence of

Answer 2

Arabinose. Activates the Arac gene

Question 3

Cells are temporarily altered so plasmid can enter

Answer 3

Be made COMPETENT

Question 4

Artificial transformation used to make cells competent

Answer 4

Calcium chloride/heat shock

Question 5

What restriction enzymes used to digest DNA

Answer 5

Eco RI, Hind III, BglIII

Question 6

Components of plasmid

Answer 6

Arabinose-Arac gene-gfp gene-amp resistance-oriC

Question 7

Tube used to purify transformed proteins

Answer 7

Hydrophobic interaction column chromatography

Question 8

Filter heat in photosynthesis lab

Answer 8

Copper sulfate

Question 9

Source of CO2 in photosynthesis lab

Answer 9

Sodium bicarbonate

Question 10

Y.G.YO.BG.YG

Answer 10

Carotene
Pleophytin
Xantophyll
Chlorophyll A
Chlorophyll B

Question 11

Pigments separated by

Answer 11

Size
Polarity
Solubility

Question 12

Enzyme activity affected by

Answer 12

PH, Temp, concentration

Question 13

HIC

Answer 13

Hydrophobic interaction column chromatography

Question 14

BEWT

Answer 14

Binding buffer exposes hydrophobic
Equilibrium buffer packs beads
Wash buffer cleans debris
TE buffer hides hydrophobic

Question 15

Starfish

Answer 15

Blastula rings of beads

Gastrula corn nut

Question 16

C-f

Answer 16

5F=9C+160