Lab #2: Microscopy

Question 1

Microscope and its Components

Answer 1

1. Eye Piece
2. Nose Piece
3. Stage
4. Slide Holder
5. Coarse Focus
6. Fine Focus
7. Stage Controls
8. Rheostat
9. Power Switch
10. Illuminator

Question 2

Eye Piece

Answer 2

Contains Ocular Lens

Magnification is 10x of the objective lens

Question 3

Nose Piece

Answer 3

Revolving component that holds each of the objectives.

Gently rotated counter clockwise, low to high magnification

Question 4

Objectives

Answer 4

4 Objectives
Receive light from the microscope’s light source.
Small and spherical
4x (Scanning), 10x, 40x, 100x

Question 5

Stage

Answer 5

Platform to secure specimen slide

Question 6

Slide Holder

Answer 6

Secure/release the specimen slide

Question 7

Coarse Focus

Answer 7

Only used at 4x (Scanning)

Used to locate the specimen of interest

Question 8

Fine Focus

Answer 8

Used at 10x 40x and 100x to refine the resolution of the image

Question 9

Stage Controls

Answer 9

Allows to move the stage and the slide forward, back, right, and left

Question 10

Rheostat

Answer 10

Adjusts the brightness of the illuminator by varying the amount of current carried to the light source

Question 11

Power switch

Answer 11

Turns the illuminator on and off

Question 12

Illuminator

Answer 12

Lightsource of the microscope

Can adjust the aperture of the illuminator to concentrate the light source

Question 13

Field of View

Answer 13

The diameter of the circle of light emitted by the illuminator

Question 14

Focus

Answer 14

The sharpness of the image. Can be focused by adjusting the coarse and fine focus knobs

Question 15

Resolution

Answer 15

The measure of image quality
The numerical aperture on the objective lens and the wavelength of light traveling through the lens
NOT MAGNIFICATION

Question 16

Contrast

Answer 16

The difference in the light and dark tones of the specimen.

It can be adjusted by changing the rheostat and illuminator aperture.

Question 17

Histology

Answer 17

Microscopic anatomy of a specific tissue/ specimen:

1. Cell
2. Tissue
3. Organ
4. Organ System

Question 18

Tissue Types

Answer 18

1. Epithelia
2. Connective
3. Muscle
4. Nervous

Question 19

Epithelia

Answer 19

Covers all inner and outer surfaces of the body
Covers most of the body’s glands
Occurs at the interfaces between 2 different physiological environments to serve as a “Boundary layer”

Question 20

Connective

Answer 20

Most diverse with several subclasses
Main Classes: Tissue proper, bone, cartilage, and blood
Connects other bodily tissues together
Establish a foundation for bone
Perform physiological duties (Storage & Delivery of nutrients)

Question 21

Muscle

Answer 21

Facilitates locomotion and other bodily movements

3 types: 1. Skeletal 2. Smooth 3. Cardiac

Question 22

Nervous

Answer 22

The brain, spinal cord, and nerves

Function to regulate and control bodily function