Lab 3 Flashcards
Evrey staining technique starts with performing what?
a bacterial smear
What bacteria did we work with in lab 3?
B. cereus
E.Coli
S.aureus
Why do we allow the smear to air dry completely before heat fixing the slide?
because if you dont allow the slide to completly air dry so the cell doesn’t lyse
What are the two types of dyes used for simple staining?
Methaline Blue
Crystal violet
A gram stain is a type of _________ staining.
A gram stain is a type of differential staining
A primary stain is used?
to stain all the cells on the slide
A decolorizing stain does what?
removes the stain from only certain types of cells
A counter stain is used?
to stain the newly decolorized cells but not effect on cells holding primary stain
How many peptidoglycan layers are in Gram - bacteria?
one layer
What was the mordant/fixative used in this lab?
iodine
What was the decolorizer used in this lab?
What was it’s effect on G + and G-
Alchohol
Gram + remained purple and Gram - became clear
What was the counterstain used in this lab?
Safranin
Fill in the blank-
Morphology Gram Status
B.Cereus
S. aureus
E.Coli
B. Cereus Rod/Bacilli Gram +
S. Aureus cocci Gram +
E. Coli Rod/Bacilli Gram -
What are the three types of selective media agar?
Phenylethyl alchohol agar
Crystal violet agar
7.5% sodium chloride agar
What are the different types of Differential/Selective Media?
Mannitol salt agar
MacConkey Agar
