Lab Exam Flashcards
What does UV light do to DNA?
It causes the formation of thymine dimers. Thymine dimers are 2 thymines next to each other instead.
What does UV light do to complimentary strands of DNA?
Forms cyclobutane ring which distorts DNA and can disrupt DNA replication as well as the formation of messenger RNA
What are the steps in dark repair?
Mammals only have dark repair. Damaged DNA is recognized. Cuts on either side of the damage and the damage is removed.polymerase 1 comes in and copies complementary strands. ligase seal ends
What enzyme is used in light repair?
Light repair is found in bacteria. There is usually an enzyme called photlyse. It uses light to break the cyclobutane rine so that thymines that we’re dimer break and hydrogen bond to adenines.
How can I light be used in health care?
It can be used to disinfect surfaces
Disinfectant versus antiseptic
Disinfectant is used on inanimate objects and antiseptics are used on the skin.
What are the targets of disinfectants ?
Disinfectants target proteins by inactivating or denaturing. They may target the membranes and that is where the electron transport chain is located so cell will die.
What are the controls of the antibiotics sensitivity test
PH, Depth of the agar, type of agar, number of bacteria on plate
What color is gram positive
Purple
What color are gram negative
Pink
What does ELISA stand for?
Enzyme linked immunobsorbant assay
What is the principle of ELISA
It is used to detect the concentration of either a antibody or antigen
What is the role of enzyme and substrate?
Enzymes take a colorless substrate and convert it into a color
What does positive and negative look like in ELISA
Positive - color production
Negative - no color production
What reasons could there be for negative test ELSIA?
Not enough antibodies or antigen produced to detect
