Laboratory Techniques

Question 1

Molecular laboratory procedure used to amplify a desired fragment of DNA

Answer 1

PCR (polymerase chain reaction)

Question 2

What are the steps of PCR?

Answer 2

1) Denature
2) Anneal
3) Elongation

Question 3

What does a Southern blot electrophorese? Northern? Western?

Answer 3

Think: SNoW DRoP

1) Southern = DNA
2) Northern = RNA
3) Western = protein

Question 4

Rapid immunologic technique testing for antigen-antibody reactivity

Answer 4

Enzyme linked Immunosorbent Assay (ELISA)

Question 5

Indirect ELISA

Answer 5

Uses test antigen to bind to a specific antibody

Question 6

Direct ELISA

Answer 6

Uses antibody to detect specific antigen

Question 7

What is the purpose of FISH?

Answer 7

To determine whether or not a gene is present

Question 8

Use of fluorescent DNA or RNA primer that binds to a specific gene site of interest on chromsome

Answer 8

Fluorescence in situ hybridization (FISH)

Question 9

How does cloning occur?

Answer 9

1) Find mRNA of interest
2) Use reverse transcriptase to produce cDNA
3) Insert cDNA into a plasmid with antibiotic resistance genes
4) Surviving bacteria indicated that the cDNA is found within

Question 10

Process where chromosomes in metaphase are stained, ordered, and numbered; Used to diagnose chromosome imbalances

Answer 10

Karyotyping